COLON CANCER BIOMARKER DISCOVERY
First Claim
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1. A method for discovering a methylation marker gene for the conversion of a normal cell to colon cancer cell comprising:
- (i) comparing converted and unconverted cell gene expression content to identify a gene that is present in greater abundance in the unconverted cell;
(ii) treating a converted cell with a demethylating agent and comparing its gene expression content with gene expression content of an untreated converted cell to identify a gene that is present in greater abundance in the cell treated with the demethylating agent; and
(iii) identifying a gene that is common to the identified genes in steps (i) and (ii), wherein the common identified gene is the methylation marker gene.
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Abstract
The present application discloses an epigenetic marker for colon cancer.
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Citations
19 Claims
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1. A method for discovering a methylation marker gene for the conversion of a normal cell to colon cancer cell comprising:
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(i) comparing converted and unconverted cell gene expression content to identify a gene that is present in greater abundance in the unconverted cell;
(ii) treating a converted cell with a demethylating agent and comparing its gene expression content with gene expression content of an untreated converted cell to identify a gene that is present in greater abundance in the cell treated with the demethylating agent; and
(iii) identifying a gene that is common to the identified genes in steps (i) and (ii), wherein the common identified gene is the methylation marker gene. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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- 16. A method of diagnosing likelihood of developing colon cancer comprising assaying for methylation of a colon cancer specific marker gene in normal appearing bodily sample.
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