Bioarray chip reaction apparatus and its manufacture
First Claim
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1. A method of making probe chips comprising the steps of:
- forming a plurality of probe arrays on a wafer;
separating said wafer into a plurality of chips, each of said chips comprising at least one probe array thereon; and
mating at least one of said chips to a package, said package comprising a reaction chamber, said reaction chamber comprising inlets for flowing fluid therein, said at least one probe array in fluid communication with said reaction chamber.
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Abstract
A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.
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Citations
49 Claims
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1. A method of making probe chips comprising the steps of:
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forming a plurality of probe arrays on a wafer;
separating said wafer into a plurality of chips, each of said chips comprising at least one probe array thereon; and
mating at least one of said chips to a package, said package comprising a reaction chamber, said reaction chamber comprising inlets for flowing fluid therein, said at least one probe array in fluid communication with said reaction chamber. - View Dependent Claims (31, 32)
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2. An array assay device, said device comprising:
- (a) a substrate receiving element for receiving a substrate having at least on array thereon, said substrate receiving element comprising a bottom surface; and
(b) a compression element for urging said bottom surface in a direction towards a substrate when present in said substrate receiving element so as to hold said bottom surface in a fixed position relative to said substrate. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 29, 30, 33, 34, 35, 36, 37, 38)
- (a) a substrate receiving element for receiving a substrate having at least on array thereon, said substrate receiving element comprising a bottom surface; and
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17. A method for performing an array assay, said method comprising:
- (a) providing an array assay device comprising;
(i) a bottom surface, (ii) a substrate receiving element for receiving a substrate having at least on array thereon, and (iii) a compression element for urging said bottom surface in a direction towards a substrate when present in said substrate receiving element so as to hold said bottom surface in a fixed position relative to said substrate;
(b) positioning a substrate comprising at least one array in said substrate receiving element;
(c) urging said bottom surface in a direction towards said positioned substrate using said compression element, whereby said bottom surface is fixed relative to said substrate present in said receiving element; and
(e) contacting a sample to said at least one array. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
- (a) providing an array assay device comprising;
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39. An apparatus for packaging a substrate, said apparatus comprising:
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a substrate having a first surface and a second surface, said first surface comprising a probe array and said second surface being an outer periphery of said first surface;
a body having a mounting surface, an upper surface, and a cavity bounded by said mounting surface and said upper surface, said second surface being attached to said cavity and said first surface being within said cavity; and
a cover attached to said mounting surface for defining an upper boundary to said cavity;
wherein said cavity comprises a diffuser and a concentrator, said diffuser and said concentrator permitting laminar fluid flow through said cavity. - View Dependent Claims (40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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Specification