Vectors for regulating gene expression
First Claim
1. A vector comprising a plurality of expression cassettes, wherein said plurality of expression cassettes comprise:
- (a) at least one gene promoting cassette comprising a first polynucleotide operably linked to a first promoter sequence; and
(b) at least one gene suppressing cassette comprising a second polynucleotide operably-linked to a second promoter sequence, wherein said second polynucleotide encodes a short interfering RNA (siRNA) molecule that reduces expression of a target gene by RNA interference.
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Abstract
The present invention pertains to vectors for regulating gene expression having at least one gene expressing cassette and at least one gene suppressing cassette, wherein the gene expression cassette encodes a polypeptide of interest, and wherein the gene suppressing cassette encodes a short interfering RNA (siRNA) molecule that reduces expression of a target gene by RNA interference. The present invention further includes vectors that contain suppressor cassettes in conjunction with cassettes upregulating gene expression regulated by either a constitutive promoter, such as a general CMV promoter, or a tissue specific promoter. The present invention further includes vectors that contain Dengue virus gene suppression cassettes. The present invention further includes pharmaceutical compositions containing such vectors, methods of modulating the expression of genes in a host using such vectors, and method of producing such vectors.
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Citations
50 Claims
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1. A vector comprising a plurality of expression cassettes, wherein said plurality of expression cassettes comprise:
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(a) at least one gene promoting cassette comprising a first polynucleotide operably linked to a first promoter sequence; and
(b) at least one gene suppressing cassette comprising a second polynucleotide operably-linked to a second promoter sequence, wherein said second polynucleotide encodes a short interfering RNA (siRNA) molecule that reduces expression of a target gene by RNA interference. - View Dependent Claims (7, 8, 10, 11, 16, 17, 22, 23, 24, 34, 36, 37, 48)
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2-6. -6. (canceled)
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9. (canceled)
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12-15. -15. (canceled)
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18-21. -21. (canceled)
- 25. A vector comprising at least one gene suppressing cassette, wherein said gene suppressing cassette comprises a polynucleotide operably-linked to a promoter sequence, wherein said polynucleotide encodes a short interfering RNA (siRNA) molecule that reduces expression of a target Dengue virus gene by RNA interference.
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31-33. -33. (canceled)
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35. (canceled)
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38-41. -41. (canceled)
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43-44. -44. (canceled)
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47. (canceled)
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50. A pharmaceutical composition comprising:
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(a) a vector and a pharmaceutically acceptable carrier, said vector comprising;
at least one gene promoting cassette comprising a first polynucleotide operably linked to a first promoter sequence; and
at least one gene suppressing cassette comprising a second polynucleotide operably linked to a second promoter sequence, wherein said second polynucleotide encodes a short interfering RNA (siRNA) molecule that reduces expression of a target gene by RNA interference;
or(b) a vector and a pharmaceutically acceptable carrier, said vector comprising;
at least one gene suppressing cassette, wherein said gene suppressing cassette comprises a polynucleotide operably linked to a promoter sequence, wherein said polynucleotide encodes a short interfering RNA (siRNA) molecule that reduces expression of a target Dengue virus gene by RNA interference.
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Specification