Nanocrystal coated surfaces
First Claim
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1. A calibration device for a fluorescence detector, said device comprising at least one deposit of essentially uniformly deposited nanocrystals.
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Abstract
The present invention provides novel calibration devices for use with fluorescent nanocrystal labels. Methods of preparing and using the calibration devices are also provided. Monodispersed populations of nanocrystals are deposited on surfaces. The monodispersed populations are obtained by dissolving the nanocrystals in a polar solvent.
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29 Claims
- 1. A calibration device for a fluorescence detector, said device comprising at least one deposit of essentially uniformly deposited nanocrystals.
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14. A process for preparing a layer of nanocrystals, said process comprising:
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i. preparing a solution of nanocrystals in a polar solvent;
ii. depositing the solution on a surface; and
iii. coating the surface with a sol-gel film. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22, 23)
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- 24. A surface comprising at least one region of dispersed nanocrystals coated with a sol-gel film.
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28. A DNA chip comprising a nanocrystal array calibration barcoding.
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29. A method of quantifying a binding reaction comprising:
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i) providing an array of an immobilized ligand on the surface of a slide;
ii) contacting the array with a sample;
iii) detecting binding of the sample to the ligand using a nanocrystal detector;
iv) comparing the signal obtained by binding of the sample to the signal generated by a series of spots, each spot comprising a predetermined standardized homogenous dispersion of nanocrystals; and
v) determining the extent of binding based on the number of nanocrystals bound to the sample spot as compared to a standardized spot.
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Specification