Method for extraction and identification of nucleic acids
First Claim
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1. A method for extracting nucleic acids from a sample comprising:
- a) obtaining a sample containing cells, viruses, or both cells and viruses;
b) adding a lysing solution comprising a detergent to the sample, thereby lysing the cells or viruses and forming a lysate;
c) adding an amount of alcohol to the lysate sufficient to aggregate or precipitate nucleic acids; and
d) purifying the nucleic acids from the lysate-alcohol mixture by filtering the mixture through a glass-fiber-filter.
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Abstract
The invention provides a method for extracting nucleic acids from a sample. The sample contains cells, viruses, or both cells and viruses. The method include adding a lysing solution containing a detergent to the sample to lyse the cells or viruses to form a lysate, adding alcohol to the lysate to aggregate or precipitate the nucleic acids, and purifying the nucleic acids from the lysate-alcohol mixture by filtering the mixture through a glass-fiber filter.
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Citations
19 Claims
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1. A method for extracting nucleic acids from a sample comprising:
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a) obtaining a sample containing cells, viruses, or both cells and viruses;
b) adding a lysing solution comprising a detergent to the sample, thereby lysing the cells or viruses and forming a lysate;
c) adding an amount of alcohol to the lysate sufficient to aggregate or precipitate nucleic acids; and
d) purifying the nucleic acids from the lysate-alcohol mixture by filtering the mixture through a glass-fiber-filter. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method for identifying a pathogen in a sample, the method comprising:
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a) obtaining a sample containing cells, viruses, or both cells and viruses;
b) adding a lysing solution comprising a detergent to the sample, thereby lysing the cells or viruses and forming a lysate;
c) adding an amount of alcohol to the lysate sufficient to aggregate or precipitate nucleic acids;
d) purifying the nucleic acids from the lysate-alcohol mixture by filtering the mixture through a glass-fiber-filter; and
e) assaying the nucleic acids to identify the pathogen.
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17. A method for identifying biological contaminants in a water sample, the method comprising:
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a) obtaining a water sample containing cells, viruses, or both cells and viruses;
b) adding a lysing solution comprising a detergent to the sample, thereby lysing the cells or viruses and forming a lysate;
c) adding an amount of alcohol to the lysate sufficient to aggregate or precipitate nucleic acids;
d) purifying the nucleic acids from the lysate-alcohol mixture by filtering the mixture through a glass-fiber-filter; and
e) assaying the nucleic acids to identify the contaminants.
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18. A method for identifying a genetic disorder in a mammal, the method comprising:
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a) obtaining a biological sample containing cells;
b) adding a lysing solution comprising a detergent to the sample, thereby lysing the cells or viruses and forming a lysate;
c) adding an amount of alcohol to the lysate sufficient to aggregate or precipitate nucleic acids;
d) purifying the nucleic acids from the lysate-alcohol mixture by filtering the mixture through a glass-fiber-filter; and
e) assaying the nucleic acids to identify the genetic disorder.
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19. A kit for extracting nucleic acids from a sample, the kit comprising:
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a) a lysing solution comprising a detergent and b) glass-fiber filters.
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Specification
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Current AssigneeNew York Blood Center Incorporated
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Original AssigneeNew York Blood Center Incorporated
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InventorsLee, Don-Hun, Pfahler, Wolfram H.E., Andrus, Linda, Prince, Alfred M.
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Application NumberUS11/336,620Publication NumberTime in Patent OfficeDaysField of SearchUS Class Current435/5CPC Class CodesC07H 21/02 with ribosyl as saccharide ...C07H 21/04 with deoxyribosyl as saccha...C12N 15/1017 by filtration, e.g. using f...C12Q 1/6806 Preparing nucleic acids for...
