Multiplex microparticle system
First Claim
1. An array of populations of microparticles, comprising a plurality of sets, wherein each set contains a plurality of populations of microparticles, wherein microparticle populations within a set are labeled with different levels of a single fluorophore such that each population exhibits a measurably distinct fluorescence intensity;
- wherein microparticles in different sets are labeled with different fluorophores;
and wherein all of said fluorophores emit light that is detectable using a single pair of detection channels, and wherein the relative emission in each of the two detection channels is distinguishably distinct between different fluorophores.
1 Assignment
0 Petitions
Accused Products
Abstract
Arrays of microparticle populations, each population labeled with a single fluorescent dye, are provided for use in multiplex assays. The populations form a virtual multidimensional array wherein each microparticle is identified by fluorescence intensity in two different fluorescence detection channels. The arrays are useful in a variety of assays, including multiplex, multi-analyte assays for the simultaneous detection of two or more analytes by, for example, flow cytometry, and a labeling reagents in, for example, microscopy. The use of singly-dyed microparticles to form multidimensional arrays greatly simplifies the creation of multiplex assays.
-
Citations
17 Claims
-
1. An array of populations of microparticles, comprising a plurality of sets,
wherein each set contains a plurality of populations of microparticles, wherein microparticle populations within a set are labeled with different levels of a single fluorophore such that each population exhibits a measurably distinct fluorescence intensity; -
wherein microparticles in different sets are labeled with different fluorophores;
and wherein all of said fluorophores emit light that is detectable using a single pair of detection channels, and wherein the relative emission in each of the two detection channels is distinguishably distinct between different fluorophores. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
-
Specification