System and method for imaging the reflectance of a substrate

• US 20060241364A1
• Filed: 04/03/2006
• Published: 10/26/2006
• Est. Priority Date: 10/01/2004
• Status: Abandoned Application

• Current Assignee: Academisch Medisch Centrum Bij De Universiteit Van Amsterdam
• Original Assignee: Academisch Medisch Centrum Bij De Universiteit Van Amsterdam
• Inventors: Ince, Can
• Application Number:
  US11/397,178
• Publication Number:
  US 20060241364A1
• Time in Patent Office: Days
• US Class Current: 600/323
• CPC Class Codes: A61B 5/0261 using optical means, e.g. infrared light
• CPC Class Codes: A61B 5/14556 by fluorescence A61B5/14555 takes precedence
• CPC Class Codes: A61B 5/412 Detecting or monitoring sepsis
• CPC Class Codes: G01N 2021/6432 Quenching
• CPC Class Codes: G01N 2021/6482 Sample cells, cuvettes
• CPC Class Codes: G01N 21/55 Specular reflectivity
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: G01N 2201/0627 Use of several LED's for spectral resolution

Abstract

The present application discloses a system and method for the comprehensive monitoring of the microcirculation in order to assess the ultimate efficacy of the cardiovascular system in delivering adequate amounts of oxygen to the organ cells. The system utilizes reflectance avoidance by reflectance filtering, such as OPS imaging or Mainstream Dark Field imaging, or by Sidestream Dark Field imaging, which utilizes external direct light on the tip of the light guide to achieve reflectance avoidance whereby incident and reflected light do not travel down the same pathway. The system can combine reflectance avoidance with other imaging modalities to provide information regarding perfusion, oxygen saturation and oxygen availability, The system can image the reflectance of a substrate and may include a light source, a light transport body configured to project light from the light source to an examination substrate and transmit light reflected by the examination substrate, and an analysis section in optical communication with the light transport body and having a reflectance avoidance imaging module and at least one of a reflectance spectrophotometry module and a fluorescence imaging module. The method may include utilizing the microcirculatory flow information, the oxygen availability information, and the adequacy of oxygenation of tissue cells information, making an early and sensitive determination regarding states of shock, such as septic, hypovolemic, cardiogenic and obstructive septic shock, in patients, and guiding resuscitation therapies aimed at correcting this condition.

Claims

1. A system for imaging the reflectance of a substrate, comprising:

a light source;

a light transport body configured to project light from the light source to an examination substrate and transmit light reflected by the examination substrate;

an analysis section in optical communication with the light transport body and having a reflectance avoidance imaging module and at least one of a reflectance spectrophotometry module and a fluorescence imaging module.

2. The system of claim 1 wherein the light transport body further comprises one or more illumination passages configured to project light from the light source and an imaging passage configured to transmit the reflected light, wherein the imaging passage is optically isolated from the one or more illumination passages.

3. The system of claim 1 wherein the analyzing section further comprises a beam director in optical communication with the light transport body.

4. The system of claim 3 wherein the beam director is configured to direct at least a portion of the light to at least one of the reflectance avoidance imaging module, the reflectance spectrophotometry module, and the fluorescence imaging module.

5. The system of claim 4 wherein the beam director is configured to be selectively actuated by a user.

6. The system of claim 3 wherein the beam director comprises at least one device selected from the group consisting of a beam splitter, dichroic junction, prism, and mirror.

7. The system of claim 1 wherein the light is selected from the group consisting of incandescent lamps, gas discharge lamps, light emitting diodes, laser diodes, gas lasers, excimer lasers, solid state lasers, chemical lasers, dye lasers, infrared sources, and UV sources.

8. The system of claim 1 further comprising a lens positioned within the imaging passage and wherein the imaging passage is in optical communication with at least one image capture device.

9. The system of claim 8 further comprising one or more optical modulators positioned within the image passage.

10. A system for imaging the reflectance of a substrate, comprising:

a light source;

a light transport body configured to project light from the light source along one or more illumination passages formed within the light transport body to an examination substrate and transmit light reflected by the examination substrate along an imaging passage which is optically isolated from the one or more illumination passages;

an analysis section in optical communication with the light transport body and comprising a reflectance avoidance imaging module.

11. The system of claim 10 wherein the analysis section further comprises at least one of a reflectance spectrophotometry module and a fluorescence imaging module.

12. The system of claim 10 wherein the one or more illumination passages are positioned radially around the imaging passage.

13. The system of claim 10 wherein the light transport body has a proximal end and a distal end and wherein the light source comprises one or more LED'"'"'s positioned at the distal end of the light transport body.

14. The system of claim 10 wherein the one or more illumination passages comprise one or more optical fibers configured to deliver illuminating energy from a remote location.

15. The system of claim 10 further comprising a device body having a proximal end and a distal end, the device body defining the one or more illumination passages and the image passages and further comprising an optically transparent disposable cap device configured to be detachably coupled to the distal end of the device body.

16. The system of claim 15 wherein the cap device comprises:

a cap body coupled to the light transport body, the cap body comprising an illumination field and an imaging relief, wherein the illumination field is positioned proximate to the light source and the imaging relief is positioned proximate to the imaging passage; and

at least one isolation surface optically isolating the illumination field from the imaging relief.

17. The system of claim 16 wherein the at least one isolation surface comprises a reflective foil which is configured to prevent light from the light source from directly entering the imaging passage without first engaging the substrate.

18. The system of claim 16 wherein the isolation surface is one of either a dye, foil or impregnation.

19. The system of claim 15 wherein the cap device is configured to detachably couple to the light transport body.

20. The system of claim 10 wherein the light source comprises LED'"'"'s of a single wavelength.

21. The system of claim 10 wherein the light source is configured to radiate light of multiple wavelengths.

22. The system of claim 10 wherein the light transport body has a proximal end and a distal end and wherein the system further comprises one or more engaging devices at the distal end of the light transport body.

23. The system of claim 22 wherein the one or more engaging devices comprises an inflatable device configured to dissipate a pressure applied to the substrate.

24. A method of imaging the reflectance of a substrate, comprising:

illuminating an examination substrate with light;

analyzing the light reflected by the examination substrate with a reflectance spectrophotometer;

determining a concentration of hemoglobin within the examination substrate based on a spectral characteristic of the examination substrate with the reflectance spectrophotometer;

analyzing the light reflected by the examination substrate with a reflectance avoidance imaging module; and

measuring a flow through a vessel within the examination substrate within a reflectance avoidance imaging module.

25. The method of claim 24 further comprising delivering a medicament or therapeutic agent to the examination substrate.

26. The method of claim 24 further comprising removing a tissue biopsy from the examination substrate.

27. A method of comprehensively monitoring the microcirculation of a patient, comprising:

illuminating a tissue substrate;

avoiding the reflection of light from the surface of the tissue substrate;

receiving light from the tissue substrate;

utilizing some of the received light to image microcirculatory flow in the tissue substrate;

utilizing some of the received light to determine oxygen availability in the microcirculation; and

utilizing some of the received light to determine the adequacy of oxygenation of the tissue cells.

28. The method of claim 27 wherein utilizing some of the received light to determine the adequacy of oxygenation of the tissue cells further comprises measuring tissue CO₂ or NADH via fluorescence imaging.

29. The method of claim 27 further comprising:

utilizing the microcirculatory flow information, the oxygen availability information, and the adequacy of oxygenation of tissue cells information;

making a determination regarding states of shock of a patient; and

guiding resuscitation therapies aimed at correcting this condition.

30. The method of claim 27 further comprising:

utilizing the microcirculatory flow information, the oxygen availability information, and the adequacy of oxygenation of tissue cells information; and

making a determination regarding cardiovascular disease and failure of the patient.

31. The method of claim 27 further comprising illuminating the tissue substrate without influencing the microcirculation.

32. The method of claim 31 further comprising providing distance between the illuminating light and the tissue substrate and avoiding pressure on the tissue substrate.

33. The method of claim 27 wherein avoiding the reflection of light from the surface of the tissue substrate is provided by reflectance filtering.

34. The method of claim 33 wherein reflectance filtering comprises one of OPS imaging or Mainstream Dark Field imaging.

35. The method of claim 27 wherein avoiding the reflection of light from the surface of the tissue substrate is provided by sidestream dark field imaging, wherein incident and reflected light do not travel down the same pathway.