Electrochemical-based analytical test strip with hydrophilicity enhanced metal electrodes

• US 20060243591A1
• Filed: 04/28/2005
• Published: 11/02/2006
• Est. Priority Date: 04/28/2005
• Status: Abandoned Application

• Current Assignee: LifeScan Scotland Limited (Johnson & Johnson)
• Original Assignee: LifeScan Scotland Limited (Johnson & Johnson)
• Inventors: Day, Richard Michael, Plotkin, Elliot V.
• Application Number:
  US11/118,266
• Publication Number:
  US 20060243591A1
• Time in Patent Office: Days
• US Class Current: 204/403.40
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: G01N 33/523 the element being adapted for a specific analyte
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose

Abstract

An electrochemical-based analytical test strip includes an electrically-insulating substrate and a metal electrode (e.g., a gold metal electrode) disposed on a surface of the electrically-insulating substrate. The metal electrode has an upper surface with hydrophilicity-enhancing chemical moieties thereon. In addition, the electrochemical-based analytical test strip also includes an enzymatic reagent layer disposed on the upper surface of the metal electrode.

Claims

1. An electrochemical-based analytical test strip comprising:

an electrically-insulating substrate;

at least one metal electrode disposed on a surface of the electrically-insulating substrate, the metal electrode having;

an upper surface with hydrophilicity-enhancing chemical moieties thereon, and

an enzymatic reagent layer disposed on the upper surface with hydrophilicity-enhancing moieties thereon.

2. The electrochemical-based analytical test strip of claim 1, wherein the at least one metal electrode is formed from at least one of gold, palladium, platinum, indium, titanium-palladium alloys and combinations thereof.

3. The electrochemical-based analytical test strip of claim 1, wherein the hydrophilicity-enhancing moieties include a thiol group.

4. The electrochemical-based analytical test strip of claim 1, wherein the at least one metal electrode is a gold metal electrode.

5. The electrochemical-based analytical test strip of claim 1, wherein the enzymatic reagent layer includes a glucose specific enzyme.

12. The electrochemical-based analytical test strip of claim 1, wherein the hydrophilicity-enhancing moieties include a disulphide group.

6. An electrochemical-based analytical test strip comprising:

an electrically-insulating substrate;

at least one gold electrode disposed on a surface of the electrically-insulating substrate, the gold metal electrode having;

an upper surface with hydrophilicity-enhancing chemical moieties thereon, and

an enzymatic reagent layer disposed on the treated upper surface, wherein the upper surface with hydrophilicity-enhancing moieties thereon is represented by;

X—

R—

S⁻

Au⁺

where;

X is either a polar side group, a positively charged side group, or negatively charged side group;

R is a carbon chain;

SH is a thiol group;

and

Au is atomic gold.

7. The electrochemical-based analytical test strip of claim 6, wherein R is a carbon chain with a length in the range of C₁ to C₅.

8. The electrochemical-based analytical test strip of claim 6, wherein X is an amine group.

9. The electrochemical-based analytical test strip of claim 6, wherein X is a carboxy group.

10. The electrochemical-based analytical test strip of claim 6, wherein X is a sulphonate group.

11. The electrochemical-based analytical test strip of claim 6, wherein the enzymatic reagent layer includes a glucose specific enzyme.