SPATIAL POSITIONING OF SPECTRALLY LABELED BEADS
First Claim
Patent Images
1. A spectral label identification method comprising:
- spatially restraining a first spectrally labeled body at a site in an array of sites;
spatially restraining a plurality of labeled bodies behind the first body so as to define an array;
dispersing a plurality of spectra from the spatially restrained bodies simultaneously across the sensor surface; and
identifying the array of bodies from the spectrum, wherein the plurality of spectrally labeled bodies are simultaneously spatially restrained as an array when the first body is restrained.
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Abstract
Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.
25 Citations
16 Claims
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1. A spectral label identification method comprising:
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spatially restraining a first spectrally labeled body at a site in an array of sites;
spatially restraining a plurality of labeled bodies behind the first body so as to define an array;
dispersing a plurality of spectra from the spatially restrained bodies simultaneously across the sensor surface; and
identifying the array of bodies from the spectrum, wherein the plurality of spectrally labeled bodies are simultaneously spatially restrained as an array when the first body is restrained. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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8. A spectral label identification method comprising:
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spatially restraining a first spectrally labeled body at a site in an array of sites;
spatially restraining a plurality of labeled bodies behind the first body so as to define an array;
sensing a first and second spectra with a scanning system by moving a sensing field between the bodies of the array; and
identifying the array of bodies from the spectrum, wherein the plurality of spectrally labeled bodies are simultaneously spatially restrained as an array when the first body is restrained. - View Dependent Claims (9, 10, 11, 12, 13)
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14. A spectral label identification method comprising:
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spatially restraining a first spectrally labeled body at a site in an array of sites;
spatially restraining an array of bodies in the array by an array of discrete binding sites, the binding sites comprising material capable of binding bodies;
sensing a first and second spectra with a scanning system by moving a sensing field between the bodies of the array; and
identifying the array of bodies from the spectrum, wherein the plurality of spectrally labeled bodies are simultaneously spatially restrained as an array when the first body is restrained.
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15. A spectral label identification method comprising:
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spatially restraining a first spectrally labeled body at a site in an array of sites;
spatially restraining an array of bodies in the array by an array of discrete binding sites, the binding sites comprising material capable of binding bodies;
dispersing a plurality of spectra from the spatially restrained bodies simultaneously across the sensor surface; and
identifying the array of bodies from the spectrum, wherein the plurality of spectrally labeled bodies are simultaneously spatially restrained as an array when the first body is restrained.
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16. A multiplexed assay system comprising:
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a plurality of bodies released in a fluid, the bodies having labels for generating identifiable spectra;
an energy transmitter coupled to the fluid so as to spatially restrain an array of bodies with a restraining energy beam; and
a sensor oriented to receive the spectrum which are simultaneously dispersed from the plurality of spatially restrained bodies across the sensor surface.
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Specification