Cellular preparation

• US 20060246043A1
• Filed: 11/10/2003
• Published: 11/02/2006
• Est. Priority Date: 04/16/2003
• Status: Abandoned Application

• Current Assignee: Creative Co., Ltd., Kazutomo Inoue
• Original Assignee: Creative Co., Ltd., Kazutomo Inoue
• Inventors: Inoue, Kazutomo, Sumi, Shoichiro, Gu, Yuanjun
• Application Number:
  US10/551,990
• Publication Number:
  US 20060246043A1
• Time in Patent Office: Days
• US Class Current: 424/93.210
• CPC Class Codes: A61K 38/39 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]

Abstract

The present invention provides a cellular preparation wherein cells capable of secreting biologically active factors such as hormones or proteins useful for patients can be retained in polyvinyl alcohol stably for a prolonged period of time and a production method of the cellular preparation, and a method for prevention and treatment of endocrine/metabolic disease, hemophilia, bone disease or cancer by administration of the cellular preparation to patients.

Claims

1. A cellular preparation comprising cells in polyvinyl alcohol admixed with a cell preservative.

2. The cellular preparation as claimed in claim 1, wherein the cell surface is further coated with an extracellular matrix.

3. The cellular preparation as claimed in claim 1 further comprising a growth factor.

4. The cellular preparation as claimed in claim 1, wherein the cells are one or two or more kinds of cells selected from the group consisting of pancreatic islet cells, pancreatic endocrine cells, hepatocytes, anterior pituitary cells, growth hormone-secreting cells, osteocytes, thyroid hormone-secreting cells, and parathyroid hormone-secreting cells.

5. The cellular preparation as claimed in claim 1, wherein the cells are treated with a cell preservative.

6. The cellular preparation as claimed in claim 1, wherein the cells are transformed cells.

7. The cellular preparation as claimed in claim 1, which has a sheet-like, plate-like, rod-like, tubular, or bead like shape.

8. The cellular preparation as claimed in claim 1, wherein the cell preservative solution is Euro-Collins solution, Cell Banker, or UW solution.

9. The cellular preparation as claimed in claim 1, which is transplanted subcutaneously, intramuscularly, or intraabdominally to mammals.

10. A method for prevention and treatment of endocrine or metabolic disease, which comprises using the cellular preparation according to claim 1.

11. The method for prevention and treatment of endocrine or metabolic disease as claimed in claim 10, wherein the endocrine or metabolic disease is diabetes or pituitary dwarfism.

12. A method for prevention and treatment of hemophilia, which comprises using the cellular preparation according to claim 1.

13. A method for prevention and treatment of bone disease, which comprises using the cellular preparation according to claim 1.

14. A method for prevention and treatment of cancer, which comprises using the cellular preparation according to claim 1.

15. A method for prevention and treatment of hepatic insufficiency or inborn errors of metabolism, which comprises using the cellular preparation according to claim 1.

17. The cellular preparation as claimed in claim 1, which is a medicine for human or animal use.

16. A method for preparing a cellular preparation, which comprises admixing a cell preservative with a polyvinyl alcohol, and mixing cells with the polyvinyl alcohol, and gelling the resulting cell-containing polyvinyl alcohol.