Oligonucleotide mimetics
First Claim
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1. A method of regulating immune function in a mammal comprising administering to said mammal an amount of an aptamer that binds CTLA-4, CD40, 4-1BB, OX40 or TGFβ
- receptor sufficient to effect said regulation.
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Abstract
The present invention relates, in general, to a method of using aptamers to modulate the immune system and, in method of inhibiting CTLR-4 function and to aptamers suitable for use in such a method.
19 Citations
21 Claims
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1. A method of regulating immune function in a mammal comprising administering to said mammal an amount of an aptamer that binds CTLA-4, CD40, 4-1BB, OX40 or TGFβ
- receptor sufficient to effect said regulation.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method of identifying an a CTLA-4 aptamer comprising:
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i) preparing a candidate mixture of nucleic acids, ii) contacting said mixture with CTLA-4, wherein nucleic acids present in said mixture having an increased affinity for CTLA-4 relative to the candidate mixture can be partitioned from the remainder of the candidate mixture, and iii) partitioning said nucleic acids having an increased affinity for CTLA-4 from the remainder of said candidate mixture. - View Dependent Claims (17)
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18. A method of identifying an aptamer that inhibits CTLA-4 function comprising assessing T cell proliferation in the presence and absence of said aptamer, enhancement of T cell proliferation in the presence of said aptamer indicating inhibition of CTLA-4 function.
- 19. An aptamer that binds CTLA-4.
Specification