Method for manufacturing an electrochemical-based analytical test strip with hydrophilicity enhanced metal electrodes

• US 20060246214A1
• Filed: 04/28/2005
• Published: 11/02/2006
• Est. Priority Date: 04/28/2005
• Status: Abandoned Application

• Current Assignee: LifeScan Scotland Limited (Johnson & Johnson)
• Original Assignee: LifeScan Scotland Limited (Johnson & Johnson)
• Inventors: Day, Richard Michael, Plotkin, Elliot V.
• Application Number:
  US11/118,265
• Publication Number:
  US 20060246214A1
• Time in Patent Office: Days
• US Class Current: 427/58
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

A method for manufacturing a portion of an electrochemical-based test strip comprising includes forming a metal electrode(s) with an upper surface on a surface of an electrically-insulating substrate. The method also includes treating the upper surface of the metal electrode(s) with a hydrophilicity-enhancing composition to form a treated upper surface on the metal electrode with hydrophilicity-enhancing chemical moieties thereon and depositing an enzymatic reagent layer on the treated upper surface of the metal electrode(s).

Claims

1. A method for manufacturing a portion of an electrochemical-based test strip, the method comprising:

forming at least one metal electrode on a surface of an electrically-insulating substrate, the at least one metal electrode having an upper surface;

treating the upper surface of the at least one metal electrode with a hydrophilicity-enhancing composition to form a treated upper surface on the metal electrode with hydrophilicity-enhancing chemical moieties thereon; and

depositing an enzymatic reagent layer on the treated upper surface of the at least one metal electrode.

2. The method of claim 1, wherein the forming step includes forming a metal electrode of at least one of gold, palladium, platinum, indium, titanium-palladium alloys and combinations thereof.

3. The method of claim 2, wherein the forming step includes forming at least one gold metal electrode.

4. The method of claim 3, wherein the treating step employs a chemical reaction of the form:

X—

R—

SH+Au→

X—

R—

S⁻

Au⁺+½

H₂

where;

X is either a polar side group, a positively charged side group, or negatively charged side group;

R is a carbon chain;

SH is a thiol group;

Au represents atomic gold;

and

X—

R—

S⁻

Au⁺ represents the gold metal electrode surface with a hydrophilicity-enhancing moiety thereon.

5. The method of claim 4, wherein X is a carbon chain in the range of C_{1 to C5}.

6. The method of claim 4, wherein X is an amine group.

7. The method of claim 4, wherein X is a carboxy group.

8. The method of claim 4, wherein X is a sulphonate group.

9. The method of claim 1, wherein the treating step spontaneously forms hydrophilicity-enhancing chemical moieties on the metal electrode in a non-self-assembled monolayer manner.

10. The method of claim 1, wherein the depositing step includes depositing an enzymatic reagent layer that includes a glucose specific enzyme.

11. The method of claim 1, wherein the treating step employs a hydrophilicity-enhancing composition that includes MESNA.

12. The method of claim 11, wherein the treating step involves dipping the at least one metal electrode in a hydrophilicity-enhancing composition that includes MESNA to spontaneously form a treated upper surface on the metal electrode with hydrophilicity-enhancing moieties thereon.

13. The method of claim 1, wherein the forming step employs a photolithographic technique.