Linker molecules for selective metallisation of nucleic acids and their uses

• US 20060246482A1
• Filed: 03/02/2006
• Published: 11/02/2006
• Est. Priority Date: 12/08/2000
• Status: Abandoned Application

• Current Assignee: Sony Deutschland GmbH (Sony Group Corp.)
• Original Assignee: Sony Limited (Sony Group Corp.)
• Inventors: Yasuda, Akio, Wessels, Jurina, Ford, William
• Application Number:
  US11/365,656
• Publication Number:
  US 20060246482A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C07C 235/74 of a saturated carbon skeleton
• CPC Class Codes: C07C 237/20 the carbon skeleton containing six-membered aromatic rings
• CPC Class Codes: C07C 323/60 with the carbon atom of at least one of the carboxyl groups bound to nitrogen atoms
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

The present invention is related to the linker molecules comprising one or more nucleic acid binding group and one or more nanoparticle binding group which are connected covalently by a spacer group. The problem underlying the present invention is to provide methods for the controlled and selective metallisation of nucleic acids, the production of nanowires which may be used, e. g., in the formation of electronic networks and circuits allowing a high density arrangement, and the components of devices that may be incorporated in such networks and circuits. This problem is solved by a linker molecule which comprises one or more nucleic acid binding group(s) and one or more nanoparticle binding group(s) which are connected covalently by a spacer group. Such linkers can be used for the manufacture of nucleic acid/linker conjugates, nanoparticle/linker conjugates, and nanoparticle/linker/nucleic acid composites and further nanowires, electronic networks, electronic circuits and junctions comprising said nanowires.

Claims

1-9. -9. (canceled)

10. A method for the manufacture of a nanoparticle comprising conjugate, wherein

a nanoparticle is combined with a linker molecule forming a nanoparticle/linker conjugate,

said linker module including one or more nucleic acid binding group(s) and one or more nanoparticle binding group(s) which are connected covalently by a spacer group.

11. A method for the manufacture of a nanoparticle-nucleic acid composite, wherein the nanoparticle/linker conjugate according to claim 10 is further reacted with a nucleic acid, forming a nanoparticle-nucleic acid composite.

12. A method for the manufacture of a nucleic acid comprising conjugate,

wherein a nucleic acid molecule is reacted with a linker molecule according to claim 10, forming a nucleic acid/linker conjugate.

13. A method for the manufacture of a nanoparticle-nucleic acid composite, wherein

the nucleic acid/linker conjugate according to claim 12 is further reacted with a nanoparticle, forming a nanoparticle-nucleic acid composite.

14. A method according to any of claims 11 to 12, characterized in that the nucleic acid is present dissolved in solution, preferably in an aqueous solution or immobilized on a substrate, preferably a non-metallic substrate or an electrode structure.

15. A method according to any of claims 11 to 12, characterized in that the nucleic acid is selected from the group comprising natural, modified, synthetic, and recombinant nucleic acids, DNA, RNA, PNA, CNA, oligonucleotides, oligonucleotides of DNA, oligonucleotides of RNA, primers, A-DNA, B-DNA, Z-DNA, polynucleotides of DNA, polynucleotides of RNA, T-junctions of nucleic acids, triplexes of nucleic acids, quadruplexes of nucleic acids, domains of non-nucleic acid polymer-nucleic acid blockcopolymers and combinations thereof.

16. A method according to any of claims 11 to 12, characterized in that the nucleic acid is double-stranded or single-stranded.

17. A method according to claim 10, characterized in that the nanoparticle is catalytically active towards electroless plating.

18. A method according to claim 10, characterized in that the nanoparticle contains a metal selected from the group comprising Fe, Co, Ni, Cu, Ru, Rh, Pd, Os, Ir, Pt, Ag, Au and combinations (e. g. alloys) of these metals.

19. A method according to claim 10, characterized in that the nanoparticle'"'"'s size is less than 10 nm.

20. A method according to claim 19, characterized in that the nanoparticle'"'"'s size is between about 0.5 nm and about 3 nm.

21. A nanoparticle/linker conjugate or nucleic acid/linker conjugate obtainable according to a method of claim 10.

22. A nanoparticle-nucleic acid composite obtainable according to a method of claim 10.

23. A method for the manufacture of a nanowire, comprising electroless deposition of a metal onto a nanoparticle-nucleic acid composite according to claim 22.

24-30. -30. (canceled)