Miniaturized genetic analysis systems and methods

• US 20060246490A1
• Filed: 04/04/2006
• Published: 11/02/2006
• Est. Priority Date: 06/29/1995
• Status: Abandoned Application

• Current Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Lipshutz, Robert J., Rava, Richard P., Fodor, Stephen P. A., Anderson, Rolfe C.
• Application Number:
  US11/396,958
• Publication Number:
  US 20060246490A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01F 2101/23 Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
• CPC Class Codes: B01F 2101/44 Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
• CPC Class Codes: B01F 31/65 the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
• CPC Class Codes: B01F 31/86 with vibration of the receptacle or part of it
• CPC Class Codes: B01F 33/25 Mixers with loose mixing elements, e.g. loose balls in a receptacle
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01F 33/3011 using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
• CPC Class Codes: B01F 33/3021 the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
• CPC Class Codes: B01F 33/403 for mixing liquids B01F33/402, B01F33/405, B01F33/406 take precedence
• CPC Class Codes: B01F 33/45 Magnetic mixers; Mixers with magnetically driven stirrers
• CPC Class Codes: B01F 33/451 wherein the mixture is directly exposed to an electromagnetic field without use of a stirrer, e.g. for material comprising ferromagnetic particles or for molten metal
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/0883 Serpentine channels
• CPC Class Codes: B01L 2300/16 Surface properties and coatings
• CPC Class Codes: B01L 2300/1827 : using resistive heater
• CPC Class Codes: B01L 2400/0481 : squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0487 : fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0655 : pinch valves
• CPC Class Codes: B01L 2400/086 : using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/5027 : by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502707 : characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 : characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502723 : characterised by venting arrangements
• CPC Class Codes: B01L 3/50273 : characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 : characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/502746 : characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: B01L 3/502753 : characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: B01L 3/502784 : specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: B01L 7/52 : with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 7/525 : with physical movement of samples between temperature zones

Abstract

The present invention provides a miniaturized integrated nucleic acid diagnostic device and system.

Claims

1. A nucleic acid extraction device, comprising:

a body having at least one chamber with at least one inlet channel; and

a porous flow-through plug disposed within the chamber, the plug having nucleic acid binding properties.

2. A method of detecting a target analyte in a plurality of samples, said method comprising:

introducing at least a first sample to a first microchannel;

introducing at least a second sample to a second microchannel;

wherein said first and second microchannel each comprise a plurality of spatially distinct regions upon which capture binding ligands are immobilized;

flowing said first and second sample through said first and second microchannels to form at least one assay complex comprising the target analyte;

recirculating the flow of at least one sample in at least one microchannel and detecting said target analyte.

3. A method according to claim 2, wherein said capture binding ligands are nucleic acids.

4. A method according to claim 2, wherein said capture binding ligands are proteins.

5. A method according to claim 2, wherein said spatially distinct regions comprise porous polymer with said capture binding ligands bound to the porous polymer.

6. A method according to claim 2, wherein said spatially distinct regions comprise beads with said capture binding ligands bound to the bead.

7. A method according to claim 2, further comprising:

selectively dispensing said capture binding ligands on spatially distinct portions of hydrogel.

8. A method according to claim 2, further comprising:

selectively dispensing said capture binding ligands on spatially distinct portions of polyacrylamide.

9. A method according to claim 2, wherein said method further comprises:

generating an optical signal indicative of the assay complex; and

wherein said detecting comprises detecting the optical signal.

10. A method according to claim 2, wherein said method further comprises:

generating a fluorescent signal indicative of the assay complex, and wherein said detecting comprises detecting the fluorescenet signale.

11. A method according to claim 2, wherein said method further comprises:

generating an electronic signal indicative of the assay complex; and

wherein said detecting comprises detecting the electronic signal.

12. A method according to claim 2, wherein said first or second microchannel is at least partially formed from a substrate comprising glass.

13. A method according to claim 2, wherein said first or second microchannel is at least partially formed from a substrate comprising plastic.

14. A method according to claim 2, wherein said first or second microchannel is at least partially formed from a substrate comprising polymer.