Method and apparatus for use in temperature controlled processing of microfluidic samples
First Claim
1. A method for processing a microfluidic sample, comprising:
- heating the microfluidic sample to a first temperature for a first predetermined time by contacting a predetermined portion of a microfluidic device with a first temperature controlled thermal element; and
heating the microfluidic sample to a second temperature for a second predetermined time by contacting the predetermined portion of the microfluidic device with a second temperature controlled thermal element.
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Abstract
Embodiments of the invention comprise microfluidic devices, instrumentation interfacing with those devices, processes for fabricating that device, and methods of employing that device to perform PCR amplification. Embodiments of the invention are also compatible with quantitative Polymerase Chain Reaction (“qPCR”) processes. Microfluidic devices in accordance with the invention may contain a plurality of parallel processing channels. Fully independent reactions can take place in each of the plurality of parallel processing channels. The availability of independent processing channels allows a microfluidic device in accordance with the invention to be used in a number of ways. For example, separate samples could be processed in each of the independent processing channels. Alternatively, different loci on a single sample could be processed in multiple processing channels.
187 Citations
25 Claims
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1. A method for processing a microfluidic sample, comprising:
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heating the microfluidic sample to a first temperature for a first predetermined time by contacting a predetermined portion of a microfluidic device with a first temperature controlled thermal element; and
heating the microfluidic sample to a second temperature for a second predetermined time by contacting the predetermined portion of the microfluidic device with a second temperature controlled thermal element. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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25-73. -73. (canceled)
Specification