Ultra-rapid DNA sequencing method with nano-transistors array based devices
First Claim
1. An apparatus for rapid DNA sequencing, including at least one channel on a substrate, wherein each channel comprising:
- a first cavity which can be dropped solution containing the single-stranded DNA molecules;
at least one nano-transistor comprising;
a semiconducting nanowire such as single-walled carbon nanotube;
a drain and source electrodes that said nanowire bridges on and can be applied an AC voltage or low DC voltage;
and a back gate for the nano-transistor that can be applied a DC bias;
at least two pairs of electrodes perpendicular to the direction of DNA molecules transportation, set in front and back of the nano-transistor to be applied AC fields to stretch the DNA molecules before and after entering into the nano-transistor, and also can be applied positive DC bias on the electrodes to let the stretched DNA molecules arrive and pass the nano-transistor from said first cavity;
a set of measuring circuit connected to the nano-transistor that could measure the current variation while the bases pass through and contact with the nanowire;
a controller that could be applied for;
controlling the sign and magnitude of the DC bias, the amplitude and frequency of the AC fields;
conditioning the measured current signal of the nano-transistor into base sequences; and
recording or comparing said base sequences with the precedent in the database of DNA molecules.
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Abstract
The invention disclosed a method based on nano-transistors for ultra-rapid DNA sequencing. The method provides micro-fabricated electrodes, which are applied for stretching and driving DNA in the solution to overpass the fabricated carbon nanotube transistors (CNTFETs) array. When DNA molecules are moved perpendicularly to the axial direction of carbon nanotubes, the DNA molecule will touch carbon nanotube surface base after base such that it can measure current flow varied from different base according to charge transfer between the DNA molecule and the nanotubes. Due to this charge-transferred mechanism, the invention could achieve DNA sequencing and make a record or compare with the precedent in the database of DNA molecules.
276 Citations
9 Claims
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1. An apparatus for rapid DNA sequencing, including at least one channel on a substrate, wherein each channel comprising:
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a first cavity which can be dropped solution containing the single-stranded DNA molecules;
at least one nano-transistor comprising;
a semiconducting nanowire such as single-walled carbon nanotube;
a drain and source electrodes that said nanowire bridges on and can be applied an AC voltage or low DC voltage;
and a back gate for the nano-transistor that can be applied a DC bias;
at least two pairs of electrodes perpendicular to the direction of DNA molecules transportation, set in front and back of the nano-transistor to be applied AC fields to stretch the DNA molecules before and after entering into the nano-transistor, and also can be applied positive DC bias on the electrodes to let the stretched DNA molecules arrive and pass the nano-transistor from said first cavity;
a set of measuring circuit connected to the nano-transistor that could measure the current variation while the bases pass through and contact with the nanowire;
a controller that could be applied for;
controlling the sign and magnitude of the DC bias, the amplitude and frequency of the AC fields;
conditioning the measured current signal of the nano-transistor into base sequences; and
recording or comparing said base sequences with the precedent in the database of DNA molecules. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method for rapid DNA sequencing with multi-channel is to apply multi-channel DNA sequencing apparatus containing at least one nano-transistor with a nano-wire, at least one set electrodes applied DC bias voltage, one set current measurement circuit and one controller, the method comprising:
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(a) Dropping ion solution containing one or multiple kinds of DNA molecules on said channel;
(b) Applying DC/AC electric field on the electrodes to let the DNA molecules be stretched straight, driven through and contact with the nanowire of the nano-transistor;
(c) Measuring the current variation of the nano-transistor while the bases of DNA molecules pass through and contact the nanowire; and
(d) Conditioning and converting the measured current signal into the base sequences, which thereby being recorded or compared with the DNA molecule database further.
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Specification