Novel method for the production of polyunsaturated fatty acids
First Claim
1. A process for the production of compounds of the following general formula I
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Abstract
The present invention relates to an improved process for the specific production of poly-unsaturated omega-3 and omega-6 fatty acids and a process for the production of triglycerides having an increased content of unsaturated fatty acids, in particular omega-3 and omega-6 fatty acids having at least two double bonds and a 20 or 22 carbon atom chain length. The invention relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, hav-ing an increased content of fatty acids, oils or lipids containing C20- or C22-fatty acids with a delta-5, 7, 8, 10 double bond, respectively due to the expression of a delta-8-desaturase and a delta-9-elon-gase from organisms such as plants preferably Algae like Isochrysis galbana or Euglena gracilis. In addition the invention relates to a process for the production of poly unsaturated fatty acids such as Eicosapentaenoic, Arachidonic, Docosapentaenoic or Doosahexaenoic acid through the co-expression of a delta-8-desaturase, a delta-9-elongase and a delta-5 desaturase in organisms such as microorganisms or plants. The invention additionally relates to the use of specific nucleic acid sequences encoding for the aforementioned proteins with delta-8-desaturase-, delta-9-elongase- or delta-5-desaturase-activity, nucleic acid constructs, vectors and organisms containing said nucleic acid sequences. The invention further relates to unsaturated fatty acids and triglycerides having an increased content of at least 1% by weight of unsaturated fatty acids and use thereof.
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Citations
25 Claims
- 1. A process for the production of compounds of the following general formula I
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10. An isolated nucleic acid sequence comprising a nucleotide sequence which encodes a Δ
- -8-desaturase selected from the group consisting of
a) a nucleic acid sequence depicted in SEQ ID NO;
1,b) a nucleic acid sequence which is derived from the sequence depicted in SEQ ID NO;
1 according to the degeneracy of the genetic code and which sequences function as a Δ
-8-desaturase. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18)
- -8-desaturase selected from the group consisting of
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11. An isolated nucleic acid sequence comprising a nucleotide sequence which encodes a Δ
- -5-desaturase selected from the group consisting of
a) a nucleic acid sequence depicted in SEQ ID NO;
5,b) a nucleic acid sequence which is derived from the sequence depicted in SEQ ID NO;
5 according to the degeneracy of the genetic code,c) derivatives of the sequence depicted in SEQ ID NO;
5 which encodes polypeptides having at least 50% homology to the sequence encoding amino acid sequences depicted in SEQ ID NO;
6 and which sequences function as a Δ
-5-desaturase. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25)
- -5-desaturase selected from the group consisting of
Specification