Flow control technique for assay devices
First Claim
1. A method for forming an assay device, the method comprising:
- applying a solvent treatment to a membrane, the solvent treatment having a sufficient dissolving capacity for the membrane so that at least one recessed region is formed therein; and
forming a detection zone on the membrane to facilitate detection of an analyte that is suspected to be present within a test sample.
2 Assignments
0 Petitions
Accused Products
Abstract
A method for controlling fluid flow in an assay device that employs a membrane is provided. Specifically, one or more recessed regions are formed in the membrane by applying a solvent treatment thereto. The solvent treatment is selected based on its particular dissolving capacity for the material used to form the membrane. For example, an alcohol-based solvent, such as methanol, may be used as a solvent for nitrocellulose membranes. Upon contact with the solvent treatment, a recessed region is formed that may serve a variety of different functions relating to flow control. In one particular embodiment, the recessed region may function as a metering channel that is capable of delivering a controlled volume of the test sample to a detection zone upon initiation of the assay.
115 Citations
34 Claims
-
1. A method for forming an assay device, the method comprising:
-
applying a solvent treatment to a membrane, the solvent treatment having a sufficient dissolving capacity for the membrane so that at least one recessed region is formed therein; and
forming a detection zone on the membrane to facilitate detection of an analyte that is suspected to be present within a test sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
-
-
18. A method for determining the presence of an analyte within a test sample, the method comprising:
-
applying a solvent treatment to a membrane, the solvent treatment having a sufficient dissolving capacity for the membrane so that at least one recessed region is formed therein; and
thereafter, contacting the metering channel with the test sample. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
-
Specification