Method of targeted gene disruption, genome of hyperthermostable bacterium and genome chip using the same
First Claim
1. A method for targeted-disruption of an arbitrary gene in the genome of a living organism, comprising the steps of:
- A) providing information of the entire sequence of the genome of the living organism;
B) selecting at least one arbitrary region of the sequence;
C) providing a vector comprising a sequence complementary to the selected region and a marker gene;
D) transforming the living organism with the vector; and
E) placing the living organism in a condition allowing homologous recombination.
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Abstract
It is intended to provide an efficient and sure gene targeting method embodied at an arbitrary position in the genome of an organism and a kit therefor. It is also intended to provide a method for targeted-disruption of an arbitrary gene in the genome of an organism which comprises: 1) the step of providing the whole sequencial data of the genome of the organism; 2) the step of selecting at least one arbitrary region in the sequence; 3) the step of providing a vector containing a sequence homologous with the region selected above and a marker gene; 4) the step of transforming the organism by the vector; and 5) the step of providing the organism under such conditions as allowing homologous recombination. Moreover, the genome of a hyperthermostable bacterium and its array are provided.
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Citations
56 Claims
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1. A method for targeted-disruption of an arbitrary gene in the genome of a living organism, comprising the steps of:
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A) providing information of the entire sequence of the genome of the living organism;
B) selecting at least one arbitrary region of the sequence;
C) providing a vector comprising a sequence complementary to the selected region and a marker gene;
D) transforming the living organism with the vector; and
E) placing the living organism in a condition allowing homologous recombination. - View Dependent Claims (2, 3, 4, 6, 7)
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5. The method according to claim 5 wherein the marker gene is located in the selected region.
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8-51. -51. (canceled)
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52. An RNAi molecule having a sequence homologous to a reading frame sequence wherein, when the reading frame of Table 2 is f-1, f-2 or f-3, the reading frame sequence has a sequence from the position of nucleic acid number (antisense strand, start) of SEQ ID NO:
- 1087 of Table 2, to the position of nucleic acid number (antisense strand, stop) or a sequence having at least 70% homology thereto.
- View Dependent Claims (53, 54, 55, 56)
Specification