Detection of human herpesviruses
First Claim
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1. A method of detecting the presence or absence of at least one human herpes virus subtype in a sample comprising;
- a) contacting said sample with first and second oligonucleotides, wherein said first and second oligonucleotides are configured to form an invasive cleavage structure with a target sequence, wherein said target sequence comprises human herpes virus sequence, and b) detecting the presence or absence of said at least one human herpes virus subtype in said sample.
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Abstract
The present invention provides methods, compositions, and kits related to nucleic acid detection assays for detecting human herpes virus. For example, the present invention provides detection assays for detecting human herpes virus subtypes HHV1- through HHV-8.
51 Citations
20 Claims
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1. A method of detecting the presence or absence of at least one human herpes virus subtype in a sample comprising;
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a) contacting said sample with first and second oligonucleotides, wherein said first and second oligonucleotides are configured to form an invasive cleavage structure with a target sequence, wherein said target sequence comprises human herpes virus sequence, and b) detecting the presence or absence of said at least one human herpes virus subtype in said sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A kit for detecting the presence or absence of at least one human herpes virus subtype in a sample comprising;
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a) first and second oligonucleotides configured to form an invasive cleavage structure with a target sequence, wherein said target sequence comprises human herpes virus sequences, and b) a cleavage agent, wherein said cleavage agent is capable of cleaving said first oligonucleotide when said cleavage structure is formed. - View Dependent Claims (18, 19, 20)
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Specification