Direct multiplex characterization of genomic DNA
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Abstract
The invention is directed to novel methods of multiplexing nucleic acid reactions, including amplification, detection and genotyping. The invention relies on the use of precircle probes that are circularized in the presence of the corresponding target nucleic acids, cleaved, and then amplified.
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Citations
58 Claims
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1-47. -47. (canceled)
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48. A composition comprising a plurality of padlock probes for detecting a plurality of target sequences in a sample, wherein each target sequence comprises first and second target domains, and each of the padlock probes comprises:
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a) a first probe sequence complementary to the first target domain;
b) a second probe sequence complementary to the second target domain;
c) a priming site that is identical for each of the plurality of probes; and
d) a restriction endonuclease site that is identical for each of the plurality of probes, wherein the restriction site occurs in a sequence intervening between the first probe sequence and the second probe sequence. - View Dependent Claims (49, 50, 51, 52, 53, 54, 55, 56, 57, 58)
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Specification