PCR FOR MRSA SCCMEC TYPING
First Claim
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1. A multiplex PCR SCCmec typing assay for Staphylococcus aureus SCCmec types I, II, III, subtypes IVa, IVb, IVc, IVd and V, and MRSA and MSSA, comprising the steps of:
- (a) obtaining an isolate of a sample of S. aureus;
(b) amplifying a loci unique to each type and subtype by a PCR technique using amplification primers selective for each of said loci; and
(c) detecting the PCR amplicons, and determining the type and subtype of the S. aureus isolate.
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Abstract
A multiplex PCR assay for the detection, identification and classification of SCCmec types and sub-types of Staphylococcal aureus has been described.
19 Citations
8 Claims
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1. A multiplex PCR SCCmec typing assay for Staphylococcus aureus SCCmec types I, II, III, subtypes IVa, IVb, IVc, IVd and V, and MRSA and MSSA, comprising the steps of:
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(a) obtaining an isolate of a sample of S. aureus;
(b) amplifying a loci unique to each type and subtype by a PCR technique using amplification primers selective for each of said loci; and
(c) detecting the PCR amplicons, and determining the type and subtype of the S. aureus isolate. - View Dependent Claims (2, 3, 4, 5)
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6. A multiplex PCR SCCmec typing assay kit for Staphylococcus aureus SCCmec types I, II, III, subtypes IVa, IVb, IVc, IVd, and V, and MRSA and MSSA, said kit comprising one or both of each of the following primer pairs:
- SEQ ID NO 1 and 2, SEQ ID NO 3 and 4, SEQ ID NO 5 and 6, SEQ ID NO 7 and 8, SEQ ID NO 9 and 10, SEQ ID NO 11 and 12, SEQ ID NO 13 and 14, SEQ ID NO 15 and 16, and SEQ ID NO 17 and 18.
- View Dependent Claims (7, 8)
Specification