Fluorescent detection system and dye set for use therewith
First Claim
1. A system comprising:
- at least two excitation sources, each excitation source adapted to provide a different excitation wavelength range than at least one other of the excitation sources;
at least one detector; and
a plurality of dyes, wherein each dye of the plurality of dyes absorbs radiation at a substantially non-overlapping peak absorption range compared to the other dyes of the plurality, each dye of the plurality emits radiation within a peak emission wavelength range that substantially overlaps a peak emission wavelength range of at least one other dye of the plurality, at least a first dye of the plurality of dyes has a first peak absorption wavelength range that overlaps with the excitation wavelength range of a first of the at least two excitation sources, and at least a second dye of the plurality of dyes has a second peak absorption wavelength range that overlaps with the excitation wavelength range of a second of the at least two excitation sources.
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Accused Products
Abstract
A system is provided that can comprise: at least two excitation sources, each adapted to provide a different excitation wavelength than at least one other; at least one detector; and a plurality of spectrally resolvable dyes. A set of dyes is also provided and can comprise one or more energy transfer dyes and each energy transfer dye can include two or more fluorescence resonance energy transfer dye moieties linked together. A set of energy transfer dyes is also provided wherein each energy transfer dye of the set comprises a different donor dye moiety than the other energy transfer dyes of the set, and the same acceptor dye moiety as the other energy transfer dyes of the set. A method of detection using the system is also provided.
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Citations
40 Claims
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1. A system comprising:
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at least two excitation sources, each excitation source adapted to provide a different excitation wavelength range than at least one other of the excitation sources;
at least one detector; and
a plurality of dyes, wherein each dye of the plurality of dyes absorbs radiation at a substantially non-overlapping peak absorption range compared to the other dyes of the plurality, each dye of the plurality emits radiation within a peak emission wavelength range that substantially overlaps a peak emission wavelength range of at least one other dye of the plurality, at least a first dye of the plurality of dyes has a first peak absorption wavelength range that overlaps with the excitation wavelength range of a first of the at least two excitation sources, and at least a second dye of the plurality of dyes has a second peak absorption wavelength range that overlaps with the excitation wavelength range of a second of the at least two excitation sources. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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- 14. A kit comprising a plurality of fluorescent dyes, wherein each dye of the plurality of fluorescent dyes absorbs radiation within a substantially non-overlapping peak absorption wavelength range compared to the other dyes of the plurality, and each dye of the plurality emits radiation within a peak emission wavelength range that substantially overlaps a peak emission wavelength range of at least one other dye of the plurality.
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30. A method comprising:
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providing a mixture comprising at least two dyes with a nucleic acid sequence-containing sample, wherein each dye of the plurality of dyes absorbs radiation within a substantially non-overlapping peak absorption wavelength range compared to the other dyes of the plurality, and each dye of the plurality emits radiation within a peak emission wavelength range that substantially overlaps a peak emission wavelength range of at least one other dye of the plurality;
irradiating the sample with a first excitation wavelength range;
detecting radiation emitted from the at least two dyes upon irradiation of the sample with the first excitation wavelength range;
irradiating the sample with a second excitation wavelength range that differs from the first excitation wavelength range; and
detecting radiation emitted from the at least two dyes upon irradiation of the sample with the second excitation wavelength range. - View Dependent Claims (31, 32, 33, 34, 35, 36, 37, 38, 39, 40)
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Specification