Electrode and sensor using same

• US 20060254909A1
• Filed: 03/16/2004
• Published: 11/16/2006
• Est. Priority Date: 03/18/2003
• Status: Abandoned Application

• Current Assignee: Tama-Tlo Corporation
• Original Assignee: Tama-Tlo Corporation
• Inventors: Maehara, Nobuyoshi, Kubo, Izumi
• Application Number:
  US10/549,479
• Publication Number:
  US 20060254909A1
• Time in Patent Office: Days
• US Class Current: 204/403.10
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/002 Electrode membranes

Abstract

To prevent an electrode improvable to measurement accuracy by using a novel self-assembled monolayer suppressing an influence of the interference substance in measuring an object to be measured, and a sensor using the same. The sensor has a modified electrode 12 and a counter electrode. The modified electrode 12 has a gold electrode 16 as an electrode base, a self-assembled monolayer 18 covering the gold electrode 16 and made of carboxyalkanethiol expressed by a chemical structural formula of HS(CH₂)_nCOOH (n=5 to 9), and an enzyme 19 immobilized on the self-assembled monolayer 18. When measuring fructose, FDH may be used as the enzyme 19. The self-assembled monolayer 18 allows Co(phen)₃²⁺ as a mediator to pass through selectivity when being formed of 7-carbxy-1-heptanethiol.

Claims

1. An electrode comprising:

an electrode base, and

a self-assembled monolayer expressed by a chemical structural formula of HS(CH₂)_nCOOH (n=5 to

     9) and covering said electrode base.

2. An electrode as set forth in claim 1, wherein an enzyme is immobilized on said self-assembled monolayer, said enzyme making an object to be measured oxidation-reduction react.

3. A sensor comprising:

a vessel receiving a sample solution in which an object to be measured dissolves, and

a modified electrode and a counter electrode to be dipped into said sample solution,

wherein said modified electrode comprises

an electrode base, and

a self-assembled monolayer expressed by a chemical structural formula of HS(CH₂)_nCOOH (n=5 to

     9) and covering said electrode base.

4. A sensor as set forth in claim 3, wherein a mediator is added into said sample solution, said mediator transferring a charge with said electrode base under said oxidation-reduction reaction of said object.

5. A sensor as set forth in claim 4, wherein a hydrophobic mediator is added into said sample solution.

6. A sensor as set forth in any one of claims 3 to 5, wherein an enzyme is immobilized on said self-assembled monolayer, said enzyme making said object oxidation-reduction react.

7. A sensor as set forth in claim 3, further comprising:

a voltage applying means for applying an electrode reaction voltage to said modified electrode, and

a calculation means for calculating concentration of said object based on an electric current flowing between said modified electrode and said counter electrode.

8. A sensor as set forth in claim 7, further comprising a reference electrode,

wherein said voltage applying means applies a predetermined voltage on the basis of a voltage of said reference electrode to said modified electrode.