SYSTEM AND METHOD FOR TREATING BIOLOGICAL TISSUE USIING CURRET ELECTRICAL FIELD

• US 20060254912A1
• Filed: 03/04/2004
• Published: 11/16/2006
• Est. Priority Date: 03/04/2003
• Status: Abandoned Application

• Current Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. (Hebrew University of Jerusalem)
• Original Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd. (Hebrew University of Jerusalem)
• Inventors: Nussinovitch, Amos
• Application Number:
  US10/547,799
• Publication Number:
  US 20060254912A1
• Time in Patent Office: Days
• US Class Current: 204/450
• CPC Class Codes: A23L 3/32 by treatment with electric currents without heating effect
• CPC Class Codes: A23L 5/41 Retaining or modifying natural colour by use of additives, e.g. optical brighteners A23L5/42 takes precedence
• CPC Class Codes: A61K 36/00 Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines antigens from pollen A61K39/36
• CPC Class Codes: C07K 1/24 by electrochemical means
• CPC Class Codes: C12M 45/07 by electrical or electromagnetic forces

Abstract

The present invention relates to a method for treating biological organic tissue, particularly plant tissue, by applying a direct current preferably of low voltage electrical field for short duration, with minimal detrimental influence on the treated tissue and to further beneficial uses of the residual tissue mass. The system and methods are useful for extraction and separation of substances of interest from the biological tissue, with optional concomitant reduction in enzyme activity, resulting in delayed or arrested browning of the tissue, and for production of imprint marks on the treated tissue. The present invention further relates to substances including pigments, minerals, oils, fragrances, vitamins, amino acids, alkaloids, extracted using the direct current treatment system, and to apparatus for performing the treatment.

Claims

1. A method for electrically extracting substances from biological matter, wherein the biological matter comprises intact tissue or crude tissue portions that undergo negligible or desirable changes in mechanical properties as a result of an electrical exposure.

2. The method of claim 1 comprising the steps of:

(i) providing intact or crude tissue in a processor assembly;

(ii) subjecting the tissue to at lease one short pulse of a direct current of low voltage resulting in a tissue extract; and

(iii) collecting the tissue extract.

3. The method of claim 2 further comprising the steps of:

(iv) removing the treated tissue; and

optionally

(v) retaining the treated tissue for further use or processing.

4. The method of claim 2 wherein the tissue is subjected to only one short pulse of a direct current of low voltage.

5. The method of any one of claims 2-4 wherein the electrical pulse is of sufficiently short duration to avoid significant temperature increase of the tissue and of the extracted material.

6. The method of claim 5 wherein the temperature increase is not more than about 0.1°

C. on average.

7. The method of claim 5 wherein the temperature increase is not more than about 4.5°

C. on average.

8. The method of any one of claims 1-7 resulting in elimination of oxidation, reduction or enzymatic activity in the treated tissue.

9. The method of any one of claims 8 resulting in delay, reduction or arrest of browning reaction of the treated tissue, compared to untreated tissue.

10. The method of any one of claims 1-7 resulting in elimination of or reduction of microorganisms present in the treated tissue, compared to untreated tissue.

11. The method of any one of claims 1-7 resulting in contraction of the tissue and decrease in its weight, compared to untreated tissue.

12. The method of claim 11 wherein the tissue is subsequently dried.

13. The method of any one of claims 1-12 wherein the tissue is provided in a solution or medium.

14. The method of claim 13 wherein the solution or medium comprise hydrophobic liquid.

15. The method of claim 14 wherein the solution or medium comprise oil.

16. The method of claim 13 wherein the solution or medium comprise hydrophilic liquid.

17. The method of any one of claims 1-12 wherein the tissue is provided without a conductive or isolating liquid.

18. The method of any one of the claims 1-12, wherein the tissue comprises at least one sample surrounded by or adjacent to at least one gel layer, that accumulates the extracted substances.

19. The method of claim 18, wherein the at least one gel layer that accumulates the substances is concentrated, dried or processed after the extraction.

20. The method of any one of claims 1-19 wherein the tissue comprises at least one sample having weight of about 0.02 g to about 10 g.

21. The method of any one of claims 1-19 wherein the tissue comprises at least one sample having a width or a Feret diameter size of about 1 mm to about 50 mm.

22. The method of any one of claims 1-21 wherein the extraction is performed using electrodes comprising a material selected from the group consisting of:

platinum, stainless steel, carbon, and gold.

23. The method of claim 22 wherein at least one of the electrodes is a platinum electrode.

24. The method of any one of claims 1-23 wherein the biological matter is a solid, a semi-solid or a gel-like tissue and the electrical exposure is performed using direct contact between at least one tissue portion and at least one of said electrodes.

25. The method of claim 24 resulting in formation of an imprint on the treated tissue, wherein the imprint becomes stable or more pronounced after drying of the tissue.

26. The method of claim 25 wherein said imprint bears the shape of the electrode used for electrification.

27. The method of any one of claims 1-26 wherein the residual tissue is left in a condition which retains mechanical properties allowing further use of said tissue.

28. The method of claim 27 wherein the further use is selected from the group consisting of food additive, cosmetics ingredient and fertilization component.

29. The method of any one of claims 1-28 wherein the tissue is a plant tissue.

30. The method of claim 29 wherein the plant tissue is selected from the group consisting of:

fruits, tubers, seeds, leaves, cotyledons, petals, and juice cells of fruits.

31. The method of claim 30 wherein the plant tissue is intact.

32. The method of claim 30 wherein the plant tissue is cut, peeled, sliced, diced or immersed.

33. The method of claim 2 wherein the direct current applied to the tissue during the extraction process is characterized by at least one parameter selected from the group consisting of:

(i) electrical current is in a range of between about 0.001 to about 0.2 A;

(ii) field strength of the electrical current is in a range of between about 0.001 to about 5 kV/cm; and

(iii) duration of the electrical exposure is in a range of between about 0.001 to about 600 seconds.

34. The method of claim 33 wherein the direct current applied to the tissue during the extraction process is characterized by at least one parameter selected from the group consisting of:

(i) the electrical current is in a range of between about 0.001 to about 0.2 A;

(ii) the field strength of the electrical current is in a range of between about 0.5 to about 5 kV/cm; and

(iii) the duration of the electrical exposure is in a range of between about 0.001 to about 10 seconds.

35. The method of claim 33 wherein the direct current applied to the tissue during the extraction process is characterized by at least one parameter selected from the group consisting of:

(i) the electrical current is in a range of between about 0.001 to about 0.2 A;

(ii) the field strength of the electrical current is in a range of between about 0.001 to about 0.5 kV/cm; and

(iii) the duration of the electrical exposure is in a range of between about 10 to about 600 seconds.

36. An extract of substances obtained from intact or crude tissue as a result of being subjected to at least one short pulse of a direct current of low voltage.

37. The extract of claim 36 produced by a method comprising the steps of:

(i) providing intact or crude tissue in a processor assembly;

(ii) subjecting the tissue to at lease one short pulse of a direct current of low voltage resulting in a tissue extract; and

(iii) collecting the tissue extract.

38. The extract of claim 37 further comprises the steps of:

(iv) removing the treated tissue; and

optionally

(v) using the treated tissue.

39. The extract of any one of claims 36-38 comprising intracellular or extracellular substances.

40. The extract of any one of claims 36-39 wherein the substances are extracted from plant tissue selected from the group consisting of roots, stems, peels, seeds, fruits, flowers and the substances are collected following the extraction.

41. The extract of any one of claims 36-40 comprising at least one pigment or mineral.

42. The extract of claim 41 wherein the at least one pigment or mineral is water-soluble.

43. The extract of claim 41 wherein the at least one pigment or mineral is not soluble in water.

44. The extract of claim 43 wherein the insoluble pigment or mineral is selected from the group consisting of carotenoids, betalaines, chlorophylls, and flavenoids.

45. An apparatus for extraction of at least one substance from intact tissue or crude tissue portions wherein the extraction method comprises the steps of:

(i) providing intact or crude tissue in a processor assembly;

(ii) subjecting the tissue to at lease one short pulse of a direct current of low voltage resulting in a tissue extract; and

(iii) collecting the tissue extract.

46. The apparatus of claim 45 wherein said method further comprises the steps of:

(iv) removing the treated tissue; and

optionally

(v) using the treated tissue.

47. The apparatus of claim 45 comprising a processor assembly including at least one processor unit having at least one electrode and a mechanism for electrifying the tissue by transmitting direct electrical current, such that the substance is released from the tissue and optionally collected.

48. The apparatus of claim 47 wherein at lease one of the electrodes comprises a material selected from the group consisting of platinum, stainless steel, carbon, and gold.

49. The apparatus of claim 48 wherein the at least one of the electrodes is a platinum electrodes.

50. An apparatus for continues extraction of substance of interest from intact tissue or crude tissue portions comprising:

i) a conveyor capable of carrying large number of cut tissue particles per minute;

ii) a metallic container into which the particles are directed;

iii) a metallic clutch capable of compressing the particles and closing the electrical circuit;

iv) a DC current supplier connected to any of the metallic components; and

v) at least one pump capable of transformation accumulated liquid or supplying new conducting or isolating fluid to the site of the electrification.