Segmentation and data mining for gel electrophoresis images
First Claim
Patent Images
1. An image and data management method, comprising the steps of:
- displaying an image;
producing, displaying, and positioning at least one graphical marker in at least one context of said image;
selecting at least one external data to associate to at least one of said graphical marker, wherein said external data is selected in one or a plurality of local or remote repositories;
associating at least one of said external data to at least one of said graphical marker and displaying a visual indication of said association; and
saving information in one or a plurality of local or remote repositories, said information comprising at least data defining said association.
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Abstract
A segmentation method is provided for the automated segmentation of spot-light structures into D images allowing precise quantification and classification of said structures and said images, based on a plurality of criteria, and further allowing the automated identification of multi-spot based patterns present in one or a plurality of images. In a preferred embodiment, the invention is used for the analysis of 2D gel electrophoresis images, with objective of quantifying protein expressions and for allowing sophisticated multi-protein pattern based image data mining, as well as image matching, registration, and automated classification.
48 Citations
23 Claims
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1. An image and data management method, comprising the steps of:
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displaying an image;
producing, displaying, and positioning at least one graphical marker in at least one context of said image;
selecting at least one external data to associate to at least one of said graphical marker, wherein said external data is selected in one or a plurality of local or remote repositories;
associating at least one of said external data to at least one of said graphical marker and displaying a visual indication of said association; and
saving information in one or a plurality of local or remote repositories, said information comprising at least data defining said association. - View Dependent Claims (2, 3, 4, 5, 6, 7, 19)
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8. A system for analyzing and managing image information, comprising:
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image input means for inputting an image;
image analysis program for automatically identifying and quantifying objects of interest within said image, said program producing image information;
association program for associating multi-source information to said image and said objects of interest, said step of associating producing associative information;
display program for displaying said image, at least some of said multi-source information, and for producing and displaying graphical information in context of said objects of interest of said image; and
storage means and program for storing said image, said image information, said graphical information, and said associative information in local or remote repositories. - View Dependent Claims (9, 20)
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10. A system for providing object-based image discovery, comprising:
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image input means for inputting an image;
image analysis program for automatically identifying and quantifying objects of interest within said image, said program producing image information, said image and said image information stored in at least one repository;
a user input means for inputting a discovery criteria;
a searching program for searching within said repositories for images that satisfy said discovery criteria; and
a display means for displaying searching results and said images. - View Dependent Claims (23)
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11. A method for automatic spot detection in digital images, comprising the steps of:
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reading an image;
computing statistical distribution of noise information in said image;
computing a multiscale analysis level N in accordance to said statistical distribution;
computing a multiscale image of said image up to said level N, and generating at least one type of regionalization of said multiscale image;
identifying objects of interest in said image in correspondence with said multiscale image and said regionalization;
identifying organized structures in said image said organized structures not objects of interest; and
characterizing and classifying said objects of interest. - View Dependent Claims (14, 15, 16, 17)
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12. A method for automatically attributing a confidence level to one or a plurality of spot objects in a digital image, comprising the steps of:
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reading an image;
automatically identifying spot objects in said image;
computing confidence level of said spot objects; and
displaying confidence level for at least one of said spot objects. - View Dependent Claims (21)
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13. A method for characterizing spot objects in an image, comprising:
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computing a multiscale representation of said image up to a level N, wherein said step of computing providing a multiscale image;
identifying and defining spot object regions on each of said levels of said multiscale image; and
linking said spot object regions identified on each of said levels of said multiscale image, said linking creating a multiscale event tree, said multiscale event tree providing information for characterizing and classifying said spot objects. - View Dependent Claims (18)
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22. A method for quantifying identified spot objects, comprising the steps of:
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computing one or a plurality of 2D diffusion functions;
fitting said diffusions functions to said identified spot objects by varying parameters of said diffusion functions in order to optimize said fitting, said parameters providing the variance, width and height of said diffusion functions;
simulating and calculating cumulative effect of said identified spot objects by means of said diffusion functions; and
quantifying said identified spot objects without said cumulative effect by means of said diffusion functions.
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Specification