Membrane assay system including preloaded particles
First Claim
1. A method of detecting an analyte in a fluid comprising:
- passing the fluid into an analyte detection device, wherein the analyte detection device comprises;
a porous membrane;
a top member positioned at a spaced distance above the porous membrane such that a first cavity is formed between the top member and the porous membrane, wherein the top member covers at least a portion of the porous membrane, and wherein the top member is substantially transparent to light;
one or more particles disposed in the first cavity, wherein each particle comprises a receptor configured to interact with the analyte, and wherein particles that interact with the analyte undergo a detectable spectroscopic change; and
a bottom member positioned below the porous membrane, wherein the bottom member is configured to receive fluid flowing through the porous membrane during use;
detecting spectroscopic signals produced by particles in the first cavity; and
determining if the analyte is present based on the detected spectroscopic signals.
2 Assignments
0 Petitions
Accused Products
Abstract
Described herein is an analyte detection device and method related to a portable instrument suitable for point-of-care analyses. In some embodiments, a portable instrument may include a disposable cartridge, an optical detector, a sample collection device and/or sample reservoir, reagent delivery systems, fluid delivery systems, one or more channels, and/or waste reservoirs. Use of a portable instrument may reduce the hazard to an operator by reducing an operator'"'"'s contact with a sample for analysis. The device is capable of obtaining diagnostic information using cellular- and/or particle-based analyses and may be used in conjunction with membrane- and/or particle-based analysis cartridges. Analytes, including proteins and cells and/or microbes may be detected using the membrane and/or particle based analysis system.
115 Citations
95 Claims
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1. A method of detecting an analyte in a fluid comprising:
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passing the fluid into an analyte detection device, wherein the analyte detection device comprises;
a porous membrane;
a top member positioned at a spaced distance above the porous membrane such that a first cavity is formed between the top member and the porous membrane, wherein the top member covers at least a portion of the porous membrane, and wherein the top member is substantially transparent to light;
one or more particles disposed in the first cavity, wherein each particle comprises a receptor configured to interact with the analyte, and wherein particles that interact with the analyte undergo a detectable spectroscopic change; and
a bottom member positioned below the porous membrane, wherein the bottom member is configured to receive fluid flowing through the porous membrane during use;
detecting spectroscopic signals produced by particles in the first cavity; and
determining if the analyte is present based on the detected spectroscopic signals. - View Dependent Claims (2, 3, 6, 18, 19, 27, 28, 29, 39, 40, 41, 42)
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4-5. -5. (canceled)
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7-17. -17. (canceled)
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20-26. -26. (canceled)
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30-38. -38. (canceled)
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43-48. -48. (canceled)
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49. An analyte detection device comprising:
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a porous membrane;
a top member positioned at a spaced distance above the porous membrane such that a first cavity is formed between the top member and the porous membrane, wherein the top member covers at least a portion of the porous membrane, and wherein the top member is substantially transparent to light;
one or more particles disposed in the first cavity, wherein each particle comprises a receptor configured to interact with the analyte, and wherein particles that interact with the analyte undergo a detectable spectroscopic change; and
a bottom member positioned below the porous membrane, wherein the bottom member is configured to receive fluid flowing through the porous membrane during use. - View Dependent Claims (69)
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50-68. -68. (canceled)
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70-84. -84. (canceled)
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85. An analyte detection system comprising:
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an analyte detection device, the analyte detection device comprising;
a porous membrane;
a top member positioned at a spaced distance above the porous membrane such that a first cavity is formed between the top member and the porous membrane, wherein the top member covers at least a portion of the porous membrane, and wherein the top member is substantially transparent to light;
one or more particles disposed in the first cavity, wherein each particle comprises a receptor configured to interact with the analyte, and wherein particles that interact with the analyte undergo a detectable spectroscopic change; and
a bottom member positioned below the porous membrane, wherein the bottom member is configured to receive fluid flowing through the porous membrane during use;
a detector optically coupled to the porous membrane, wherein the detector is configured to view at least a portion of the membrane through the window; and
a fluid delivery system coupled to the analyte detection device, wherein the fluid delivery system is configured to deliver fluid samples to the analyte detection device. - View Dependent Claims (93, 94)
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86-92. -92. (canceled)
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95-128. -128. (canceled)
Specification