Polymer-coated substrates for binding biological molecules
First Claim
1. A substrate for supporting a biological array, the substrate comprising:
- a reactive surface to which a polymer coating can attach by covalent bonds;
an even coating of a polymer containing functional groups, which can reduce nonspecific binding of various biomolecules to a polymer-coated substrate surface.
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Abstract
A substrate, which that is capable of attaching biomolecules, and a method for preparing the substrate are provided. The substrate has a reactive surface that can covalently attach a polymer coating containing functional groups, which can reduce nonspecific binding of biomolecules to the surface for a biological array. Optionally, at least a portion of the substrate may be coated with an intermediate tie layer, which enhances the covalent bonding between the polymer coating with the underlying substrate. The present invention also pertains to a method that uses electrostatic blocking agents to reduce non-specific binding of proteins to a substrate, especially anhydride-modified surfaces.
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Citations
103 Claims
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1. A substrate for supporting a biological array, the substrate comprising:
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a reactive surface to which a polymer coating can attach by covalent bonds;
an even coating of a polymer containing functional groups, which can reduce nonspecific binding of various biomolecules to a polymer-coated substrate surface. - View Dependent Claims (2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 101, 102, 103)
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3. (canceled)
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35-61. -61. (canceled)
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62. A method for making a biological array, the method comprising:
- providing a substrate;
preparing a reactive surface on said substrate for attaching a polymer coating;
applying a polymer coating to the reactive surface of the substrate; and
, depositing biomolecules onto said polymer-coated surface.
- providing a substrate;
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63-100. -100. (canceled)
Specification