Magnetically-assisted test strip cartridge and method for using same
First Claim
Patent Images
1. A magnetically-assisted test strip apparatus comprising:
- a cartridge;
a channel running within said cartridge;
a sample port in communication with said channel, wherein said sample port allows introduction of a fluid sample into said channel; and
said channel containing an aptamer.
3 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides a small (business card-sized) disposable mesofluidic or microfluidic plastic cartridge containing several straight microchannels potentially filled with culture media, solubilizing reagents (e.g., detergents) nitrocellulose paper strip, gel or other matrix materials and lyophilized paramagnetic microbeads or microparticles coated with antibodies, nucleic acid aptamers, oligonucleotides, or other types of proteins or other receptors for capture, concentration and ultrasenstive detection of target analytes in environmental, food, animal, or clinical body fluid samples.
-
Citations
24 Claims
-
1. A magnetically-assisted test strip apparatus comprising:
-
a cartridge;
a channel running within said cartridge;
a sample port in communication with said channel, wherein said sample port allows introduction of a fluid sample into said channel; and
said channel containing an aptamer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
-
-
19. The method of effecting a sandwich assay in a magnetically-assisted test strip apparatus, comprising:
-
introducing a target analyte into a channel in said magnetically-assisted test strip apparatus, said magnetically-assisted test strip apparatus comprising a cartridge, said channel running within said cartridge, a sample port in communication with said channel, wherein said sample port allows introduction of a fluid sample containing said target analyte into said channel, said channel containing a magnetic bead, a receptor bound to said magnetic bead, said receptor being a antibody, aptamer, or oligonucleotide, and a detection window;
effecting a first reaction wherein said target analyte binds to said receptor bound to said magnetic bead;
passing magnetic field along length of channel in order to influence said magnetic bead to move along channel to a location viewable through said detection window;
effecting a second reaction wherein said target analyte binds with a reagent, said reagent being chosen from the group;
quantum dot, fluorophore-filled nanoparticle, fluorescein-filled nanoparticle, ruthenium trisbipyridine-filled nanoparticle, fluorescent protein, FRET, molecular beacon, colloidal gold, enzyme, labeled antibody, or aptamer;
analysis by means of visual assessment, fluorometer, fluorescence intensity, spectrofluorometry features, fluorescence lifetime analysis, spectrophotometry, spectrofluorometer, time-resolved fluorometer, photodiode, photodiode array, charge-couple device camera, complementary metal oxide semiconductor, photomultiplier tube, or other photodetection device that interfaces with said detection window. - View Dependent Claims (20, 21, 22, 23, 24)
-
Specification