Device and Method to Facilitate Directed Delivery and Electroporation

• US 20060260003A1
• Filed: 01/17/2006
• Published: 11/16/2006
• Est. Priority Date: 07/16/2003
• Status: Abandoned Application

• Current Assignee: University of South Florida (State University System of Florida)
• Original Assignee: University of South Florida (State University System of Florida)
• Inventors: Jaroszeski, Mark J., Heller, Richard, Gilbert, Richard
• Application Number:
  US11/306,945
• Publication Number:
  US 20060260003A1
• Time in Patent Office: Days
• US Class Current: 800/278
• CPC Class Codes: A61N 1/0416 Anode and cathode
• CPC Class Codes: A61N 1/0424 Shape of the electrode
• CPC Class Codes: A61N 1/325 for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body electrodes for external use A61N1/0428
• CPC Class Codes: A61N 1/327 for enhancing the absorption properties of tissue, e.g. by electroporation
• CPC Class Codes: C12M 35/02 Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
• CPC Class Codes: C12N 15/87 Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Abstract

The present invention provides an apparatus and method that will accomplish molecular delivery and subsequent electroporation with a single apparatus without the need for separate molecule injection and placement of the electroporation applicators.

Claims

1. A molecular transport and electroporation apparatus comprising:

a reservoir having an electrically conductive top end cap and a micro-porous bottom end cap; and

the bottom end cap further comprising at least one electroporation applicator.

2. The apparatus of claim 1, further comprising an electrical insulator positioned to electrically isolate the conductive top end cap from the reservoir.

3. The apparatus of claim 1, further comprising a polarization supply voltage in circuit communication with the top end cap.

4. The apparatus of claim 1, wherein the micro-porous bottom end cap further comprises a micro-porous membrane;

and

the at least one electroporation applicator is embedded in the micro-porous membrane of the bottom end cap.

5. The apparatus of claim 1, wherein the electroporation applicators are non-penetrating.

6. The apparatus of claim 1, wherein the at least one electroporation applicator further comprises a plurality of electroporation applicators.

7. The apparatus of claim 1, further comprising an electroporation supply voltage in circuit communication with the at least one electroporation applicator.

8. The apparatus of claim 1, further comprising a target molecule positioned within the reservoir.

9. The apparatus of claim 8, wherein the target molecule is a protein.

10. The apparatus of claim 8, wherein the target molecule is a plasmid.

11. The apparatus of claim 8, wherein the target molecule is a therapeutic drug.

12. The apparatus of claim 1, wherein the reservoir further comprises a plurality of isolated compartments.

13. The apparatus of claim 12, wherein each of the plurality of isolated compartments further comprises a target molecule positioned within each of the isolated compartments of the reservoir.

14. The apparatus of claim 12, wherein the top end cap of the reservoir further comprises a plurality of electrically isolated portions, each electrically isolated portion of the end cap associated with each of the plurality of isolated compartments.

15. A molecular transport and electroporation apparatus comprising:

a reservoir having an electrically conductive top end cap and a micro-porous bottom end cap;

the bottom end cap further comprising at least one electroporation applicator;

electrical insulator positioned to electrically isolate the conductive top end cap from the reservoir;

a polarization supply voltage in circuit communication with the top end cap;

at least one non-penetrating electroporation applicator integral to the bottom end cap;

an electroporation supply voltage in circuit communication with the at least one electroporation applicator.

16. The apparatus of claim 15, further comprising a target molecule positioned within the reservoir.

17. The apparatus of claim 15, wherein the target molecule is a protein.

18. The apparatus of claim 15, wherein the target molecule is a plasmid.

19. The apparatus of claim 15, wherein the target molecule is a therapeutic drug.

20. The apparatus of claim 15, wherein the reservoir further comprises a plurality of isolated compartments.

21. The apparatus of claim 20, wherein each of the plurality of isolator compartments further comprises a target molecule positioned within each of the isolated compartments of the reservoir.

22. The apparatus of claim 20, wherein the top end cap of the reservoir further comprises a plurality of electrically isolated portions, each electrically isolated portion of the end cap associated with each of the plurality of isolated compartments.

23. A method for molecular delivery and electroporation, the method comprising the steps of:

containing a target molecule within a reservoir;

contacting a micro-porous bottom end cap of the reservoir to a surface of a cellular tissue;

applying a polarization voltage to a conductive top end cap of the reservoir adapted to transport the target molecule through the micro-porous bottom end cap of the reservoir and diffuse the target molecule across the surface of the cellular tissue; and

applying an electroporation voltage to at least one electroporation applicator positioned on the micro-porous bottom end cap to introduce the target molecule into the interior of a cell of the cellular tissue.

24. The method of claim 23, wherein the step of applying a polarization voltage further comprises applying a polarization voltage following a predetermined protocol.

25. The method of claim 23, wherein the step of applying an electroporation voltage further comprises applying an electroporation voltage following a predetermined protocol.

26. The method of claim 23, further comprising the step of altering the electrochemical potential of the target molecule upon application of the polarization voltage to the conductive top end cap.

27. A method for molecular delivery and electroporation, the method comprising the steps of:

containing at least two distinct target molecules in a reservoir, each of the distinct target molecules contained within an isolated compartment of the reservoir;

applying a polarization voltage to at least an isolated portion of a conductive top end cap of the reservoir, each of the isolated portions of the top end cap associated with each of the isolated compartments of the reservoir, the application of the polarization voltage adapted to transport the target molecule through the micro-porous bottom end cap of the reservoir and diffuse the target molecule across the surface of the cellular tissue; and

applying an electroporation voltage to at least one electroporation applicator positioned on the micro-porous bottom end cap to introduce the target molecule into the interior of a cell of the cellular tissue.

28. The method of claim 27, wherein the step of applying a polarization voltage further comprises applying a polarization voltage following a predetermined protocol.

29. The method of claim 27, wherein the step of applying an electroporation voltage further comprises applying an electroporation voltage following a predetermined protocol.

30. The method of claim 27, further comprising the step of altering the electrochemical potential of the target molecule upon application of the polarization voltage to the conductive top end cap.