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Method of transforming soybean

  • US 20060260012A1
  • Filed: 06/17/2003
  • Published: 11/16/2006
  • Est. Priority Date: 06/22/2002
  • Status: Abandoned Application
First Claim
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1. A method for transforming soybean cells or tissue, comprising:

  • (a) preparing an explant from a soybean seed by. (i) removing a hypocotyl from said soybean seed;

    (ii) removing one cotyledon along with its adjacent axillary bud, leaving primary leaves attached to a remaining cotyledon; and

    (iii) removing a portion of a primary leaf from said remaining cotyledon, thereby generating a primary leaf base; and

    (b) co-cultivating said explant with Agrobacterium comprising at least one nucleic acid of interest to be incorporated into a genome of one or more soybean cells.

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