Method of transforming soybean
First Claim
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1. A method for transforming soybean cells or tissue, comprising:
- (a) preparing an explant from a soybean seed by. (i) removing a hypocotyl from said soybean seed;
(ii) removing one cotyledon along with its adjacent axillary bud, leaving primary leaves attached to a remaining cotyledon; and
(iii) removing a portion of a primary leaf from said remaining cotyledon, thereby generating a primary leaf base; and
(b) co-cultivating said explant with Agrobacterium comprising at least one nucleic acid of interest to be incorporated into a genome of one or more soybean cells.
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Abstract
The present disclosure provides methods for Agrobacterium-mediated transformation of soybean cells or tissue and regeneration of the transformed cells or tissue into transformed plants. The methods may be used for transforming many soybean cultivars.
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Citations
22 Claims
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1. A method for transforming soybean cells or tissue, comprising:
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(a) preparing an explant from a soybean seed by. (i) removing a hypocotyl from said soybean seed;
(ii) removing one cotyledon along with its adjacent axillary bud, leaving primary leaves attached to a remaining cotyledon; and
(iii) removing a portion of a primary leaf from said remaining cotyledon, thereby generating a primary leaf base; and
(b) co-cultivating said explant with Agrobacterium comprising at least one nucleic acid of interest to be incorporated into a genome of one or more soybean cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 19, 20)
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18. A method for producing a stably transformed soybean plant, comprising:
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(a) preparing an explant from a soybean seed by;
(i) removing a hypocotyl from said soybean seed;
(ii) removing one cotyledon along with its adjacent axillary bud, leaving primary leaves attached to a remaining cotyledon; and
(iii) removing a portion of each primary leaf from said remaining cotyledon, thereby generating a pair of primary leaf bases;
(b) co-cultivating said explant with Agrobacterium comprising a nucleic acid of interest to be incorporated into a genome of a soybean cell;
(c) inducing shoot formation from each primary leaf base;
(d) cultivating at least one formed shoot in a medium containing a selection agent;
(e) selecting a transformed shoot; and
(f) regenerating a selected transformed shoot into a soybean plant. - View Dependent Claims (21, 22)
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Specification