Blood micro-separator

• US 20060263265A1
• Filed: 05/23/2005
• Published: 11/23/2006
• Est. Priority Date: 05/23/2005
• Status: Abandoned Application

• Current Assignee: Chung-Shan Institute of Science & Technology Armaments Bureau MND (Government of The Republic of China)
• Original Assignee: Chung-Shan Institute of Science & Technology Armaments Bureau MND (Government of The Republic of China)
• Inventors: Wu, Hsien-Ming, Huang, Ker-Jer, Chan, Chih-Chun, Kang, Der-Ren, Yang, Gwei-Chu
• Application Number:
  US11/135,849
• Publication Number:
  US 20060263265A1
• Time in Patent Office: Days
• US Class Current: 422/101
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2300/0806 Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28

Abstract

A micro-separator for separating red blood cells from blood serum in a blood sample is provided. The main mechanism driving the separation of the blood sample is centrifugal force. With physical mechanisms including surface tension and functional microstructures, the red blood cells and the blood serum in a small volume of blood (about 25 micro-liters) are separated. The entire micro-separation apparatus is disposed on a disk-shaped structure. The micro-separator can integrate with other micro-devices such as micro-mixers, micro-distributors or micro-reactors to form a fully functional apparatus. Combining the rotary driver of a CD player and an optical inspection device with a signal processing system, an inexpensive, fully automatic, parallel-processing inspection system is produced. The micro-separator has a simple structure with no movable components, and its separation mechanism does not depend on either filter papers or micro-pore structures so blockage is prevented and it can easily combine with other microfluidic devices.

Claims

1. A blood micro-separator suitable for a disk-type biochemical analysis, comprising:

a blood injection well;

an auxiliary filling well;

a blood separation well;

a barrier block within the blood separation well; and

a valve and two overflow channels.

2. The micro-separator of claim 1, wherein the barrier block within the blood separation well can separate the separated red blood cells and blood serum from the liquid coming from the auxiliary filling well so that blood serum contamination can be prevented and the liquid from the filling well can be smoothly injected into the blood separation well.

3. The micro-separator of claim 1, wherein the barrier block inside the blood separation well has a small distance (about tens of micrometers) from the top ceiling to serve as an air vent for balancing out fluid pressure.

4. The micro-separator of claim 1, wherein the storage capacity of the auxiliary filling well and the blood separation well has to be calculated so the total volume of the auxiliary filling well and the blood separation well up to the liquid surface at the overflow channel inlet is equal to the volume of blood injected into the micro-separator, and the storage capacity of the auxiliary filling well is roughly 40% of that of the blood separation well.

5. The micro-separator of claim 1, wherein the separator comprises a valve to trigger a rotation speed higher than the speed necessary for separating the red blood cells from the blood serum in a blood specimen.

6. The micro-separator of claim 1, wherein the micro-separator is fabricated using a polydimethylsiloxane (PDMS) material or using a processed hydrophobic material.