METHODS FOR DETECTING TARGET NUCLEIC ACIDS USING COUPLED LIGATION AND AMPLIFICATION

• US 20060263794A1
• Filed: 03/23/2006
• Published: 11/23/2006
• Est. Priority Date: 05/30/2000
• Status: Abandoned Application

• Current Assignee: Applera Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applera Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Wenz, Hans Michael
• Application Number:
  US11/277,348
• Publication Number:
  US 20060263794A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6855 Ligating adaptors
• CPC Class Codes: C12Q 1/6862 Ligase chain reaction [LCR]
• CPC Class Codes: C12Q 2525/155 incorporating/generating a new priming site

Abstract

The present invention relates to the detection of nucleic acid sequences using coupled ligation and amplification. The coupling of ligation and amplification allows multiplex detection of nucleic acid sequences. The invention also relates to methods, reagents, and kits that employ addressable-support specific sequences in detecting nucleic acid sequences.

Claims

1-114. -114. (canceled)

1. A method for detecting one or more target sequences in a sample comprising:

combining the sample with a probe set for each target sequence, the probe set comprising (a) at least one first probe, comprising a target-specific portion and a 5′

primer-specific portion, and (b) at least one second probe, comprising a target-specific portion and a 3′

primer-specific portion, wherein the probes in each set are suitable for ligation together when hybridized adjacent to one another on a complementary target sequence, and wherein at least one probe in each probe set further comprises an addressable support-specific portion located between the primer-specific portion and the target-specific portion;

to form a ligation reaction mixture;

subjecting the ligation reaction mixture to at least one cycle of ligation, wherein adjacently hybridizing complementary probes are ligated to one another to form a ligation product comprising the 5′

primer-specific portion, the target-specific portions, at least one addressable support-specific portion, and the 3′

primer-specific portion;

combining the ligation reaction mixture with;

(a) at least one primer set, the primer set comprising (i) at least one primer comprising the sequence of the 5′

primer-specific portion of the ligation product, and (ii) at least one second primer comprising a sequence complementary to the 3′

primer-specific portion of the ligation product, and (b) a polymerase, to form an amplification reaction mixture;

subjecting the amplification reaction mixture to at least one cycle of amplification to generate one or more amplification products;

combining the one or more amplification products with at least two different sequence-specific mobility-modifiers, wherein each different mobility-modifier is capable of sequence-specific binding to a different addressable support-specific portion and comprises (a) a tag complement for specifically binding the addressable support-specific portion of one of the one or more amplification products, and (b) a tail which imparts to each mobility modifier a mobility that is distinctive relative to the mobilities of one or more other of said at least two different mobility-modifiers in a mobility-dependent analysis technique;

removing mobility-modifiers that are not sequence-specifically bound to the one or more amplification products from mobility-modifiers that are sequence-specifically bound to the one or more amplification products;

releasing the sequence-specifically bound mobility-modifiers from the one or more amplification products;

subjecting the released mobility-modifiers to a mobility-dependent analysis technique; and

detecting the one or more target sequences in the sample by detecting distinctive positions of the mobility-modifiers.

2. The method of claim 1, wherein at least one sequence-specific mobility modifier comprises a label.

3. The method of claim 1, wherein the mobility-dependent analysis technique is electrophoresis.