Macroporous support for chemical amplification reactions
First Claim
1. A method for amplifying nucleic acids in pores of a two-dimensionally designed macroporous support material which includes first and second surfaces lying opposite each other, wherein a multiplicity of discrete pores with a diameter in the range of from 500 nm to 100 μ
- m, an aspect ratio of pore depth to pore aperture of at least 10;
1, and a pore density of from 104 to 108/cm2 are arranged distributed over an entire surface region, wherein the method comprises the steps of;
(a) providing a predetermined part of a reaction mixture suitable for the amplification reaction in at least one region of the support material which includes at least two pores, such that the part of the reaction mixture is bound non-covalently to the pore wall of the support material so that a reaction region is thereby simultaneously defined on the support material surface;
(b) adding a sample over the entire support material, the sample containing the part of the reaction mixture necessary for the completion of an amplification reaction;
(c) conducting the amplification reaction; and
(d) detecting at least one amplification product.
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Accused Products
Abstract
A method for carrying out an amplification of nucleic acids in pores of a two-dimensionally designed macroporous support material according to one embodiment includes the step of providing a predetermined part of a reaction mixture necessary for the amplification of a nucleic acid in pores of the support material. A device for carrying out the amplification of nucleic acids according to one embodiment includes a two-dimensionally designed macroporous support material having a multiplicity of pores, wherein a predetermined part of a reaction mixture for carrying out an amplification of nucleic acids is provided in the pores.
16 Citations
14 Claims
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1. A method for amplifying nucleic acids in pores of a two-dimensionally designed macroporous support material which includes first and second surfaces lying opposite each other, wherein a multiplicity of discrete pores with a diameter in the range of from 500 nm to 100 μ
- m, an aspect ratio of pore depth to pore aperture of at least 10;
1, and a pore density of from 104 to 108/cm2 are arranged distributed over an entire surface region, wherein the method comprises the steps of;
(a) providing a predetermined part of a reaction mixture suitable for the amplification reaction in at least one region of the support material which includes at least two pores, such that the part of the reaction mixture is bound non-covalently to the pore wall of the support material so that a reaction region is thereby simultaneously defined on the support material surface;
(b) adding a sample over the entire support material, the sample containing the part of the reaction mixture necessary for the completion of an amplification reaction;
(c) conducting the amplification reaction; and
(d) detecting at least one amplification product. - View Dependent Claims (2, 3, 4)
- m, an aspect ratio of pore depth to pore aperture of at least 10;
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5. A device comprising a two-dimensionally designed macroporous support material which includes first and second surfaces lying opposite each other, wherein a multiplicity of discrete pores with a diameter in the range of from 500 nm to 100 μ
- m, an aspect ratio of pore depth to pore aperture of at least 10;
1, and a pore density of from 104 to 108/cm2 are arranged and distributed over an entire surface region, wherein the device comprises at least one region in which a predetermined part of a reaction mixture for carrying out an amplification of nucleic acids is bound non-covalently in at least two pores. - View Dependent Claims (6, 7, 8, 9, 10)
- m, an aspect ratio of pore depth to pore aperture of at least 10;
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11. A method for amplification of nucleic acids that includes incorporating into a device a two-dimensionally designed macroporous support material which comprises first and second surfaces lying opposite each other, wherein a multiplicity of discrete pores with a diameter in the range of from 500 nm to 100 μ
- m, an aspect ratio of pore depth to pore aperture of at least 10;
1, and a pore density of from 104 to 108/cm2 are arranged and distributed over an entire surface region. - View Dependent Claims (12, 13, 14)
- m, an aspect ratio of pore depth to pore aperture of at least 10;
Specification