Antibody protein analysis chip

• US 20060263826A1
• Filed: 05/18/2006
• Published: 11/23/2006
• Est. Priority Date: 05/18/2005
• Status: Abandoned Application

• Current Assignee: Nuclea Biomarkers, LLC
• Original Assignee: Nuclea Biomarkers, LLC
• Inventors: Muraca, Patrick J.
• Application Number:
  US11/436,246
• Publication Number:
  US 20060263826A1
• Time in Patent Office: Days
• US Class Current: 435/7.100
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: G01N 33/54386 Analytical elements

Abstract

The various embodiment described herein disclose an antibody protein analysis chip. The analysis chip comprises a substrate having a plurality of layers. At least one layer is a conductive layer comprising a conductive material. The analysis chip also comprises a main channel (straight-line and S-shaped configurations) etched in the conductive layer wherein the channel begins at a loading reservoir and ends at an outlet channel. A plurality of side channels are etched in the conductive layer. The side channels are in communication with the main channel and end in a well. A plurality of wells of the analysis chip comprise at least one protein-specific antibody. In addition, a kit is disclosed comprising an embodiment of the antibody protein analysis chip.

Claims

1. An antibody protein analysis chip comprising:

a substrate comprising a plurality of layers wherein at least one layer is a conductive layer comprising a conductive material;

a main channel etched in the conductive layer which begins at a loading reservoir and ends at an outlet channel;

a plurality of side channels etched in the conductive layer at a substantially perpendicular angle to the main channel wherein the side channels are in communication with the main channel and end in at least one well; and

a protein-specific antibody confined in at least one well.

2. The analysis chip of claim 1 wherein the conductive material is gold.

3. The analysis chip of claim 1 wherein the conductive material is aluminum.

4. The analysis chip of claim 1 wherein the conductive material is chromium.

5. The analysis chip of claim 1 wherein the protein-specific antibody is engaged to a detectable tag.

6. The analysis chip of claim 5 wherein the detectable-tag is a quantum dot.

7. The analysis chip of claim 1 wherein the antibody is specific for a kinase peptide.

8. The analysis chip of claim 1 wherein the substrate is further engaged to a hydrophobic ink layer.

15. The analysis chip of claim 1 wherein the antibody is specific for a kinase peptide.

16. The analysis chip of claim 1 wherein the substrate is further engaged to a hydrophobic ink layer.

9. A antibody protein analysis chip comprising:

a substrate comprising a plurality of layers wherein at least one layer is a conductive layer comprising a conductive material;

an S-shaped channel etched in the conductive layer which begins at a loading reservoir and ends at an outlet channel;

a plurality of side channels etched in the conductive layer wherein the side channels are in communication with the main channel and end in at least one well;

a protein-specific antibody confined in at least one well; and

a battery in electrical communication with the substrate.

10. The analysis chip of claim 9 wherein the conductive material is gold.

11. The analysis chip of claim 9 wherein the conductive material is aluminum.

12. The analysis chip of claim 9 wherein the conductive material is chromium.

13. The analysis chip of claim 9 wherein the protein-specific antibody is engaged to a detectable tag.

14. The analysis chip of claim 13 wherein the detectable-tag is a quantum dot.

17. A kit comprising:

an antibody protein analysis chip comprising;

a substrate comprising a plurality of layers wherein at least one layer is a conductive layer comprising a conductive material;

an S-shaped channel etched in the conductive layer which begins at a loading reservoir and ends at an outlet channel;

a plurality of side channels etched in the conductive layer wherein the side channels are in communication with the main channel and end in a well;

an at least one protein-specific antibody confined in each well; and

a battery in electrical communication with the substrate; and

a cover engaged to the analysis chip wherein the cover comprises a plurality of openings corresponding to the wells.

18. The kit of claim 17 wherein the conductive material of the analysis chip is gold.

19. The kit of claim 17 wherein the antibodies in each well are engaged to a detectable tag.

20. The kit of claim 19 wherein the detectable tag is a quantum dot.