Efficient haploid cell sorting flow cytometer systems
8 Assignments
0 Petitions
Accused Products
Abstract
A flow cytometry system (1) for sorting haploid cells, specifically irradiatable sperm cells, with an intermittingly punctuated radiation emitter (56). Embodiments include a beam manipulator (21) and even split radiation beams directed to multiple nozzles (5). Differentiation of sperm characteristics with increased resolution may efficiently allow differentiated sperm cells to be separated higher speeds and even into subpopulations having higher purity.
165 Citations
176 Claims
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1-59. -59. (canceled)
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60. A method of flow cytometry sample processing comprising the steps of:
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establishing at least one sheath fluid;
flowing said at least one sheath fluid into at least two nozzles;
injecting at least one irradiatable sample into said at least one sheath fluid;
utilizing at least one shared resource to process said at least one irradiatable sample;
subjecting said irradiatable sample to radiation;
exciting said irradiatable sample with said radiation;
emitting fluorescence from said excited sample;
detecting an amount of said emitted fluorescence from each particle in said sample;
evaluating said amount of emitted fluorescence from each particle in said sample;
selecting an electrical condition to be associated with each particle of said sample in said sheath fluid flow;
charging a stream of said irradiatable sample and sheath fluid based upon deduced properties of each particle of said sample in said sheath fluid flow;
forming a charged drop;
isolating said charged drop from said sheath fluid flow;
deflecting said charged drops;
sorting said sample; and
collecting said sorted sample. - View Dependent Claims (67, 68, 69, 71, 72, 73, 74, 76, 77, 78, 79, 80, 81, 84, 89, 90, 91, 92, 97, 98, 99, 100, 101, 102, 105, 106, 107, 108, 109, 110, 111, 112, 114, 115, 116, 118, 119, 120, 122, 123, 124, 125, 126)
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61-66. -66. (canceled)
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70. (canceled)
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75. (canceled)
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82-83. -83. (canceled)
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85-88. -88. (canceled)
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93-96. -96. (canceled)
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103-104. -104. (canceled)
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113. (canceled)
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117. (canceled)
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121. (canceled)
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127-174. -174. (canceled)
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175. A flow cytometry system comprising:
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at least one sheath fluid port to introduce a sheath fluid;
at least one sample injection element having an injection point through which an irradiatable sample may be introduced into said sheath fluid;
at least two nozzles located in part below said at least one injection point;
an oscillator to which said sheath fluid is responsive;
a radiation emitter;
a particle sample fluorescence detector;
a processing unit connected to said particle sample fluorescence detector;
a drop charge circuit to apply an electrical condition to a stream of said irradiatable sample cells and sheath fluid;
a first and second deflection plate each disposed on opposite sides of a free fall area in which a drop forms, wherein said first and second deflection places are oppositely charged; and
a particle sample collector.
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176-231. -231. (canceled)
Specification