Methods and systems relating to embryonic stem cell lines

• US 20060263879A1
• Filed: 12/22/2005
• Published: 11/23/2006
• Est. Priority Date: 12/30/2004
• Status: Abandoned Application

• Current Assignee: Stemlifeline, Inc.
• Original Assignee: Stemlifeline, Inc.
• Inventors: Foulk, Russell A., Simon Valles, Carlos, Krtolica, Ana
• Application Number:
  US11/317,887
• Publication Number:
  US 20060263879A1
• Time in Patent Office: Days
• US Class Current: 435/366
• CPC Class Codes: C12N 2500/02 Atmosphere, e.g. low oxygen conditions
• CPC Class Codes: C12N 2500/90 Serum-free medium, which may still contain naturally-sourced components
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2502/243 Cells of the female genital tract, non-germinal ovarian cells
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696
• CPC Class Codes: G16B 50/00 ICT programming tools or database systems specially adapted for bioinformatics
• CPC Class Codes: G16B 50/30 Data warehousing; Computing architectures
• CPC Class Codes: G16B 50/40 Encryption of genetic data

Abstract

The invention provides methods for generating customized hESC and lines thereof for future use in the treatment of genetic relations. In one aspect, the customized hESC lines are grown using feeder cells from biologically or genetic relations in order to avoid contamination by foreign pathogens or exposure to foreign antigens.

Claims

1. A method for establishment and use of a human embryonic stem cell line comprising

generating a human embryonic stem cell line;

storing the human embryonic stem cell line in connection with a database for directing use of the human embryonic stem cell line in treating a familial subject.

2. The method of claim 1, wherein generating the human embryonic stem cell line comprises

culturing, on or in the presence of human feeder cells or human serum, a human blastocyst generated using a female donor egg, to generate a human embryonic stem cell line,

wherein the human feeder cells or human serum are derived from a biological or genetic relation of the blastocyst and the feeder cells are mitotically inactivated.

3. The method of claim 1, wherein the human embryonic stem cell line and the database are stored in a human embryonic stem cell line bank.

4. The method of claim 1, wherein the database contains an information record corresponding to the human embryonic stem cell line.

5. The method of claim 3, wherein the database contains an information record corresponding to the human embryonic stem cell line.

6. The method of claim 5, further comprising storing the human feeder cells in the bank and listing the human feeder cells in the information record, wherein the human feeder cells are derived from a biological or genetic relation of the human embryonic stem cell line and were used to generate the human embryonic stem cell line.

7. The method of claim 5, further comprising storing the human serum in the bank and including the human serum in the information record, wherein the human serum is derived from a biological or genetic relation of the human embryonic stem cell line and was used to generate the human embryonic stem cell line.

14. The method of claim 1, wherein the database is a computer database.

15. The method of claim 3, wherein the information record comprises a feeder cell sample field or a serum sample field.

16. The method of claim 4, wherein the information record comprises a feeder cell sample field or a serum sample field.

17. The method of claim 3, wherein the information record comprises a stem cell line generation protocol field.

18. The method of claim 4, wherein the information record comprises a stem cell line generation protocol field.

19. The method of claim 3, wherein the information record comprises a stem cell line profile field.

20. The method of claim 4, wherein the information record comprises a stem cell line profile field.

21. The method of claim 3, wherein the information record comprises storage location information and an identification code.

25. The method of claim 3, further comprising levying a charge for each cryopreserved sample based on length of storage.

28. The method of claim 1, wherein the human embryonic stem cell line used to treat a familial subject is not screened for pathogen content prior to such use.

30. The method of claim 3, wherein the stem cell bank comprises a plurality of samples of human feeder cells.

31. The method of claim 3, wherein the stem cell bank comprises a plurality of samples of human serum.

32. The method of claim 2, wherein the human blastocyst is generated by culturing a zygote in the presence of endometrial epithelial cells.

34. The method of claim 1, wherein the human embryonic stem line is generated by the method comprising

culturing, on human feeder cells, a human blastocyst generated using a female donor egg, to generate a human embryonic stem cell line,

wherein the human feeder cells are generic human feeder cells and are mitotically inactivated.

8-12. -12. (canceled)

22-24. -24. (canceled)

26-27. -27. (canceled)

29. (canceled)

33. (canceled)

35. A computer readable medium or combination of computer-readable media, containing a program for maintaining a computer-based human embryonic stem cell line database, the program comprising code to effect:

storing information about a human embryonic stem cell line; and

storing an information record in a database.

36-38. -38. (canceled)

39. A method for providing human embryonic stem cell line information to a client, the method comprising computer-implemented steps of:

storing information about a human embryonic stem cell line; and

storing an information record or records that corresponds to the identity and offspring of a donor of the egg and optionally a donor of the sperm in a database.

40. A computer readable medium having computer readable signals stored thereon, the signals defining instructions that, as a result of being executed by a computer, control the computer to perform a process for providing human embryonic stem cell line information, the process comprising acts of:

storing information about a human embryonic stem cell line; and

storing an information record or records that corresponds to the identity and offspring of a donor of the egg and optionally a donor of the sperm in a database.