DIFFERENTIAL WHITE BLOOD COUNT ON A DISPOSABLE CARD

• US 20060263888A1
• Filed: 12/30/2005
• Published: 11/23/2006
• Est. Priority Date: 06/02/2000
• Status: Abandoned Application

• Current Assignee: Honeywell International Inc.
• Original Assignee: Honeywell International Inc.
• Inventors: Padmanabhan, Aravind, Fritz, Bernard S., Reutiman, Peter
• Application Number:
  US11/306,508
• Publication Number:
  US 20060263888A1
• Time in Patent Office: Days
• US Class Current: 436/63
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0636 Focussing flows, e.g. to laminate flows
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0605 check valves
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502776 specially adapted for focusing or laminating flows
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 2015/016 White blood cells
• CPC Class Codes: G01N 2015/1486 Counting the particles
• CPC Class Codes: G01N 33/5002 Partitioning blood components

Abstract

An apparatus and approach for performing multipart differentiation such as, for example, three, four and five part differentiation of white blood cells in a card or cartridge. The may be a combination of several kinds of lysing and/or staining/marking in a variety of sequential and parallel runs to achieve the differentiation of the cells. Various cell counts may also be performed.

Claims

1. A fluid analysis apparatus comprising:

a disposable card;

an optical fluidic channel situated in the card;

a first coupling region situated in the card;

a fluid sample source connected to the first coupling region;

a lysing reagent source connected to the first coupling region;

a lysing channel connected to the first coupling region;

a sheath reagent source;

a second coupling region connected the lysing channel and to the sheath reagent source;

an optical fluidic channel connected to the second coupling region; and

a light source and detector proximate to the optical fluidic channel.

2. The apparatus of claim 1, wherein the second coupling region is a hydrodynamic focusing chamber.

3. The apparatus of claim 1, wherein light scatter measurements from the detector provide three part differentiation (lymphocytes, monocytes and neutrophils) of white blood cells.

4. The apparatus of claim 3, further comprising a stain agent source connected to the first coupling region.

5. The apparatus of claim 4, wherein the detector provides light absorption or light fluorescence measurements for a fourth part (eosinophil) and fifth part (basophil) differentiation of the white blood cells.

6. The apparatus of claim 3, wherein the light scatter measurements provide a fourth part (eosinophil) differentiation of the white blood cells.

7. The apparatus of claim 6, wherein the light source and detector provide light absorption or light fluorescence measurements for a fifth part (basophil) differentiation of the white blood cells.

8. The apparatus of claim 3, wherein the light scatter measurements provide a white blood cell count.

9. The apparatus of claim 3, further comprising a selective lysing reagent source connected to the first coupling region.

10. The apparatus of claim 9, wherein the light scatter measurements provide a fourth part (eosinophil) differentiation of the white blood cells.

11. The apparatus of claim 10, further comprising a second selective lysing reagent source connected to the first coupling region.

12. The apparatus of claim 11, wherein the light scatter measurements provide a fifth part (basophil) differentiation of the white blood cells.

13. The apparatus of claim 12, wherein the light measurements are possible from all angles including ninety degrees.

14. The apparatus of claim 3, wherein the lysing reagent source provides lysing reagent and stain agent to the first coupling region for lysing and staining blood cells.

15. The apparatus of claim 14, wherein the light source and detector provide light absorption or light fluorescence measurements for a fourth part (eosinophil) differentiation of white blood cells.

16. The apparatus of claim 15, wherein the light source and detector provide fluorescence measurements for a fifth part (basophil) differentiation of white blood cells.

17. The apparatus of claim 15, further comprising:

a selective lysing reagent connected to the first coupling region; and

wherein the light source and detector provide scatter measurements for a fifth part (basophil) differentiation of the white blood cells.

18. The apparatus of claim 3, further comprising:

a stain agent source connected to the second coupling region; and

the light source and detector provide a fourth part (eosinophil) and fifth part (basophil) differentiation of the white blood cells.

19. The apparatus of claim 5, wherein the disposable card is a microfluidics based card.

20. The apparatus of claim 5, wherein the apparatus meets wavier features of the FDA clinical laboratory improvement amendments.

21. The apparatus of claim 5, wherein the disposable card is manufactured with a lamination process.

22. The apparatus of claim 5, wherein the disposable card is manufactured with an injection molding process.

23. The apparatus of claim 5, wherein the disposable card comprises plastic.

24. The apparatus of claim 5, wherein the disposable card comprises glass.

25. The apparatus of claim 5, wherein the disposable card comprises plastic and glass and/or a hybrid mixture thereof.

26. The apparatus of claim 5, wherein the sources are situated in the disposable card.

27. The apparatus of claim 5, wherein the sources are situated off the disposable card.

28. A method for measurement in a disposable card, comprising:

providing a blood sample;

lysing red blood cells of the blood sample;

focusing white blood cells into a single file;

providing light to the white blood cells; and

obtaining parameters of the white blood cells from light scattered by the white blood cells.

29. The method of claim 28, wherein the parameters are possibly from scattered light from all angles including ninety degrees.

30. The method of claim 28, wherein the parameters comprise a four-part (lymphocyte, monocyte, neutrophil and eosinophil) differentiation of the white blood cells.

31. The method of claim 30, wherein the parameters further comprise a fifth part (basophil) differentiation of the white blood cells.

32. The method of claim 28, wherein the parameters comprise a white blood cell count.

33. The method of claim 28, wherein the parameters comprise a three part (lymphocyte, monocyte and neutrophil) differentiation of the white blood cells.

34. The method of claim 33, further comprising a first selective lysing of the blood to obtain a fourth part (eosinophil) differentiation of the white blood cells.

35. The method of claim 34, comprising a second selective lysing of the blood to obtain a fifth part (basophil) differentiation of the white blood cells.

36. The method of claim 33, wherein:

staining the white blood cells; and

further comprising obtaining additional parameters from light absorption or light fluorescence from stain on the white blood cells; and

wherein the additional parameters comprise a fourth (eosinophil) part differentiation of the white blood cells.

37. The method of claim 36, wherein the additional parameters comprise a fifth (basophil) part differentiation of the white blood cells.

38. The method of claim 36, further comprising a selective lysing of the blood cells to obtain a fifth part (basophil) differentiation of the white blood cells.

39. The method of claim 33, further comprising:

staining the blood; and

obtaining additional parameters from light absorption or light fluorescence from stain on the white blood cells; and

wherein the additional parameters comprise a fourth part (eosinophil) differentiation of the white blood cells.

40. The method of claim 39, wherein the additional parameters further comprise a fifth part (basophil) differentiation of the white blood cells.

41. The method of claim 33, further comprising:

staining the white blood cells;

obtaining additional parameters from light fluorescence or light absorption from stain on the white blood cells; and

wherein the additional parameters comprise a fourth part (eosinophil) differentiation of the white blood cells.

42. The method of claim 41, wherein the additional parameters further comprise a fifth part (basophil) differentiation of the white blood cells.

43. The method of claim 38, wherein the disposable card is a microfluidics based card.

44. The method of claim 38, wherein the method meets wavier features of the FDA clinical laboratory improvement amendments.

45. The method of claim 38, wherein the disposable card is manufactured with a lamination process.

46. The method of claim 38, wherein the disposable card is manufactured with an injection molding process.

47. The method of claim 38, wherein the disposable card comprises plastic.

48. The method of claim 38, wherein the disposable card comprises glass.

49. The method of claim 38, wherein the disposable card comprises plastic and glass and/or a hybrid mixture thereof.