Electroporation controlled with real time imaging

• US 20060264752A1
• Filed: 03/13/2006
• Published: 11/23/2006
• Est. Priority Date: 04/27/2005
• Status: Abandoned Application

• Current Assignee: Oncobionic Inc. (AngioDynamics, Inc.), Regents of the University of California (University of California)
• Original Assignee: And Oncobionic, Regents of the University of California (University of California)
• Inventors: Mikus, Paul, Onik, Gary, Rubinsky, Boris
• Application Number:
  US11/375,600
• Publication Number:
  US 20060264752A1
• Time in Patent Office: Days
• US Class Current: 600/439
• CPC Class Codes: A61B 18/1477 Needle-like probes
• CPC Class Codes: A61B 2018/00577 Ablation
• CPC Class Codes: A61B 2018/00613 Irreversible electroporation reversible electroporation A61N1/327
• CPC Class Codes: A61B 2018/00755 Resistance or impedance
• CPC Class Codes: A61B 2018/00875 Resistance or impedance
• CPC Class Codes: A61B 5/0536 Impedance imaging, e.g. by tomography
• CPC Class Codes: A61B 8/0833 involving detecting or locating foreign bodies or organic structures
• CPC Class Codes: A61B 8/4416 related to combined acquisition of different diagnostic modalities, e.g. combination of ultrasound and X-ray acquisitions
• CPC Class Codes: A61N 1/0412 Specially adapted for transcutaneous electroporation, e.g. including drug reservoirs
• CPC Class Codes: A61N 1/327 for enhancing the absorption properties of tissue, e.g. by electroporation

Abstract

A method and a system for producing the method are disclosed whereby irreversible electroporation pulses are produced across an area of target tissue. A medical imaging device is used to create an image of the irreversible electroporation in real time thereby making it possible to determine the area of electroporation and the extent of results obtained and to adjust the positioning of electrodes and/or the current as needed based on the image being viewed.

Claims

1. A method, comprising the steps of:

identifying a target tissue area;

placing a first electrode and a second electrode such that the target tissue area is positioned between the first electrode and the second electrode;

applying a pulse of current between the first electrode and the second electrode; and

creating an image of an area of the target tissue between the first electrode and the second electrode.

2. The method of claim 1, wherein creating an image is carried out using ultrasound.

3. The method of claim 1, wherein the pulse is applied for a duration in a range of from about 1 microsecond to about 62 seconds.

4. The method of claim 1, wherein a plurality of pulses are applied for a period of about 100 microseconds, ±

about 10 microseconds.

5. The method of claim 2, wherein from about 1 to about 15 pulses are applied.

6. The method of claim 2, wherein about eight pulses of about 100 microseconds each in duration are applied.

7. The method of claim 4, wherein the pulses produce a voltage gradient in a range of from about 50 volt/cm to about 8000 volt/cm.

8. The method of claim 1, wherein the first electrode is placed at about 5 mm to 10 cm from the second electrode and the first and second electrodes are placed using an image created of the target area.

9. The method of claim 1, wherein the target tissue area is a tumor.

10. The method of claim 1, further comprising:

adjusting current-to-voltage ratio based on the image.

11. The method as claimed in claim 1, wherein the pulse of current between the first electrode and the second electrode is applied in a sufficient voltage, current, period of time and number of times so as to obtain irreversible electroporation of cells in the target tissue area without causing damage to tissue surrounding the target tissue area.

12. A method of ablating undesirable tissue, comprising:

(a) identifying an area of a tissue as a target for destruction;

(b) applying a current to obtain electroporation of the area;

(c) imaging the area as an indication of degree of electroporation;

(d) adjusting a determined magnitude of the applied voltage in accordance with imaging to achieve irreversible electroporation of the identified area as the target of destruction.

13. A method of treating cancer, comprising:

(a) identifying a grouping of biological cells in a tissue of a living mammal as being cancer cells and applying a voltage across the cells;

(b) imaging the cells as an indication of degree of electroporation of the biological cells; and

(c) adjusting a determined magnitude of the applied voltage in accordance with images to achieve an irreversible electroporation of the cells identified as being cancer cells.

14. The method of claim 13, wherein step (b) comprises continuously imaging to obtain an indication of onset of electroporation of biological cells, and step (c) comprises adjusting the duration of the applied voltage in accordance with continuously obtained images.

15. A method of irreversible electroporation comprising the steps of:

(a) identifying a target tissue area;

(b) placing a monitoring device into the tissue in the area of the identified target tissue;

(c) placing electrodes in a manner such that the identified target tissue area is positioned between the electrodes;

(d) applying a test current which test current is insufficient to cause irreversible electroporation;

(e) monitoring the test current effects on the target tissue and at a remote location;

(f) extrapolating back based on the amount of the test current to determine an amount of current necessary to achieve irreversible electroporation; and

(g) applying current so as to obtain irreversible electroporation.

16. The method of claim 15, wherein the monitoring device is a high impedance needle.