Portable fluorescence scanner for molecular signatures

• US 20060264761A1
• Filed: 03/15/2006
• Published: 11/23/2006
• Est. Priority Date: 03/18/2005
• Status: Abandoned Application

• Current Assignee: Siemens AG
• Original Assignee: Siemens AG
• Inventors: Strob, Wolfgang, Knoche, Jochem
• Application Number:
  US11/375,934
• Publication Number:
  US 20060264761A1
• Time in Patent Office: Days
• US Class Current: 600/476
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/415 the glands, e.g. tonsils, adenoids or thymus
• CPC Class Codes: A61B 5/418 lymph vessels, ducts or nodes
• CPC Class Codes: A61B 5/7207 of noise induced by motion artifacts
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging

Abstract

A fluorescence scanner, having a light source for generating excitation light, a detector for detecting fluorescent light, and a data acquisition unit. The excitation light source is operated in pulsed fashion. The pulsed mode results in short exposure times for the fluorescence images, so that artifacts caused by motion are reduced.

Claims

1. An imaging device, comprising:

an excitation light source;

a fluorescence detector; and

a data acquisition unit connected to the fluorescence detector,

wherein the excitation light source is operable in pulsed fashion.

2. The imaging device of claim 1, wherein the fluorescence detector has a filter operable to attenuate visible light.

3. The imaging device of claim 2, wherein the filter is disposed outside the optical path between a fluorescent material and the fluorescence detector and inserted in the optical path during operation of the pulsed excitation light source.

4. The imaging device of claim 1, further comprising a trigger, the excitation light source being pulsed by actuation of the trigger.

5. The imaging device of claim 4, wherein actuating the trigger initiates one light pulse from the excitation light source.

6. The imaging device of claim 1, wherein the duration of an operating pulse of the excitation light source is approximately 300 ms or less.

7. The imaging device of claim 1, wherein the excitation light source comprises a light emitting diode (LED), a laser diode, a halogen light bulb or combinations thereof.

8. The imaging device of claim 1, wherein the excitation light source is disposed to emit excitation light at an angle of between approximately 30°

and approximately 45°

to an optical primary axis of the fluorescence detector.

9. The imaging device of claim 1, wherein the excitation light source is operable to generate optical energy in a wavelength range of between approximately 700 nm and approximately 800 nm.

10. The imaging of claim 1, wherein the fluorescence detector is operable to detect fluorescence at wavelengths longer than approximately 700 nm.

11. The imaging device of claim 10, wherein the fluorescence detector is operable to detect fluorescence at a wavelength of approximately 780 nm

12. The imaging device of claim 1, wherein the fluorescence detector and the light source are connected to a battery contained in, or attached to, the imaging device.

13. The imaging device of claim 12, wherein image data obtained by the fluorescence detector is transmitted by modulation of data on a carrier wave, using a carrier wave in the radio frequency or optical frequency range.