Nanoscale sensors

• US 20060269927A1
• Filed: 05/25/2005
• Published: 11/30/2006
• Est. Priority Date: 05/25/2005
• Status: Abandoned Application

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Patolsky, Fernando, Lieber, Charles M., Zheng, Gengfeng
• Application Number:
  US11/137,784
• Publication Number:
  US 20060269927A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 2563/125 the label being enzymatic, i.e. proteins, and non proteins, such as nucleic acid with enzymatic activity
• CPC Class Codes: C12Q 2563/155 Particles of a defined size, e.g. nanoparticles
• CPC Class Codes: C12Q 2565/607 being a sensor, e.g. electrode

Abstract

Various aspects of the present invention generally relate to nanoscale wire devices and methods for use in determining analytes suspected to be present in a sample, and systems and methods of immobilizing entities such as reaction entities relative to nanoscale wires. In one aspect, a nucleic acid, such as DNA, may be immobilized relative to a nanoscale wire, and in some cases, grown from the nanoscale wire. In certain embodiments, the nucleic acid may interact with entities such as other nucleic acids, proteins, etc., and in some cases, such interactions may be reversible. As an example, an enzyme such as telomerase may be allowed to bind to DNA immobilized relative to a nanoscale wire. The telomerase may extend the length of the DNA, for instance, by reaction with free deoxynucleotide triphosphates in solution; additionally, various properties of the nucleic acid may be determined, for example, using electric field interactions between the nucleic acid and the nanoscale wire. In another aspect, the invention provides systems and methods for attaching entities such as nucleic acids, receptors such as gangliosides, or surfactants to a nanoscale wire, for example, using aldehyde-producing reactions or hydrophobic interactions. In some aspects, certain systems and methods of the present invention may be used to determine an analyte suspected to be present in a sample, for example, a toxin or a small molecule. Systems and methods of using such nanoscale wires are disclosed in other aspects of the invention, for example, within a microarray. Still other aspects of the invention include assays, sensors, kits, and/or other devices that include such nanoscale wires, methods of making and/or using functionalized nanoscale wires (for example, in drug screening or high-throughput screening), and the like.

Claims

1. A method, comprising acts of:

exposing a sample suspected of containing a nucleic acid synthesis enzyme to a nucleic acid immobilized relative to a nanoscale wire; and

determining binding of the enzyme to the nucleic acid.

2. The method of claim 1, wherein the nucleic acid synthesis enzyme is a telomerase.

3. The method of claim 1, wherein the nucleic acid comprises DNA.

4. The method of claim 1, wherein the enzyme comprises a nucleic acid complementary to at least a portion of the nucleic acid immobilized relative to the nanoscale wire.

5. The method of claim 1, comprising diagnosing cancer based on the binding of the telomerase to the nucleic acid.

6. The method of claim 1, comprising determining an electrical property of the nanoscale wire to determine binding of the telomerase to the nucleic acid.

7. The method of claim 1, further comprising causing the enzyme to add one or more nucleotides to the nucleic acid.

8. The method of claim 1, comprising exposing the enzyme to one or more deoxynucleotide triphosphates.

9. The method of claim 1, wherein the sample comprises a cell lysate.

10. A method, comprising an act of:

adding one or more nucleotides to a nucleic acid immobilized relative to a nanoscale wire.

11. The method of claim 10, wherein the act of adding comprises adding one or more nucleotides to the nucleic acid using a nucleic acid synthesis enzyme.

12. The method of claim 11, wherein the enzyme is a telomerase.

13. The method of claim 11, wherein the enzyme is a DNA polymerase.

14. The method of claim 10, wherein the act of adding comprises adding a telomeric repeat sequence to the nanoscale wire.

15. The method of claim 14, wherein the telomeric repeat sequence is TTAGGG (SEQ ID NO:

1).

16. The method of claim 10, wherein the nucleic acid comprises DNA.

17. The method of claim 10, wherein the nucleic acid comprises RNA.

18. The method of claim 10, wherein the nucleic acid is covalently bonded to the nanoscale wire.

19. The method of claim 18, wherein the nucleic acid is covalently bonded to the nanoscale wire via an imine bond.

20-26. -26. (canceled)

27. An article, comprising:

a nanoscale wire;

a first nucleic acid immobilized relative to a nanoscale wire; and

a second nucleic acid immobilized relative to the first nucleic acid.

28-129. -129. (canceled)