Dendrimer-based DNA extraction methods and biochips

• US 20060269961A1
• Filed: 08/10/2006
• Published: 11/30/2006
• Est. Priority Date: 12/16/2003
• Status: Abandoned Application

• Current Assignee: Tadashi Matsunaga, Yokogawa Electric Corporation
• Original Assignee: Tadashi Matsunaga, Yokogawa Electric Corporation
• Inventors: Matsunaga, Tadashi, Satou, Saya, Fukushima, Kazuhisa, Takeyama, Haruko
• Application Number:
  US11/501,900
• Publication Number:
  US 20060269961A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C12Q 1/682 Signal amplification
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2565/501 being an array of oligonucleotides
• CPC Class Codes: C12Q 2565/629 being a microfluidic device
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

The present invention provides a dendrimer-based biochip, wherein a flow channel through which a solution containing biopolymer molecules is flowed is formed in the substrate of the biochip, a plurality of dendrimer molecules one end of each of which is bound to the walls of the flow channel are formed thereon, and probe biopolymer or antibody molecules are bound to the tips of the dendrimer molecules so that, if the probe biopolymer molecules are bound, then target biopolymer molecules can be captured by means of a complementary combination and, if the antibody molecules are bound, then protein can be extracted by means of antigen-antibody reaction, whereby biopolymers can be retrieved in a highly efficient manner.

Claims

1. A dendrimer-based biochip, wherein a flow channel through which a solution containing biopolymer molecules is flowed, is formed in the substrate of said biochip, a plurality of dendrimer molecules, one end of each of which is bound to the walls of said flow channel, are formed thereon, probe biopolymer or antibody molecules are bound to the tips of said dendrimer molecules and, if said probe biopolymer molecules are bound, then target biopolymer molecules are captured by means of a complementary combination and, if said antibody molecules are bound, then protein is extracted by means of antigen-antibody reaction.

2. The dendrimer-based biochip of claim 1, wherein said dendrimer is a multibranched polyamide amine dendrimer produced by providing aminosilane treatment on the walls of said flow channel and overlaying a film of amidoamine, which is produced by the reaction of methyl acrylate with ethylenediamine, upon the aminosilane-treated area as a dendron unit.

3. The dendrimer-based biochip of claim 1, wherein the generation of said dendrimer molecules is second or higher.

4. The dendrimer-based biochip of claim 2, wherein the generation of said dendrimer molecules is second or higher.

5. The dendrimer-based biochip of claim 1, wherein concavities and convexities are formed on the walls of said flow channel.

6. The dendrimer-based biochip of claim 2, wherein concavities and convexities are formed on the walls of said flow channel.

7. The dendrimer-based biochip of claim 3, wherein concavities and convexities are formed on the walls of said flow channel.