Biomarkers sensing

• US 20060270919A1
• Filed: 05/09/2006
• Published: 11/30/2006
• Est. Priority Date: 05/11/2005
• Status: Abandoned Application

• Current Assignee: Mytek, LLC (Ams-Osram AG)
• Original Assignee: Mytek, LLC (Ams-Osram AG)
• Inventors: Brenner, Mary K.
• Application Number:
  US11/430,436
• Publication Number:
  US 20060270919A1
• Time in Patent Office: Days
• US Class Current: 600/310
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: A61K 49/0058 Antibodies
• CPC Class Codes: G01N 2021/6417 Spectrofluorimetric devices
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: H01L 2224/45124 Aluminium (Al) as principal constituent
• CPC Class Codes: H01L 2224/45144 Gold (Au) as principal constituent
• CPC Class Codes: H01L 2224/48091 Arched
• CPC Class Codes: H01L 2224/48227 connecting the wire to a bond pad of the item
• CPC Class Codes: H01L 24/45 of an individual wire connector
• CPC Class Codes: H01L 2924/00 Indexing scheme for arrangements or methods for connecting or disconnecting semiconductor or solid-state bodies as covered by H01L24/00
• CPC Class Codes: H01L 2924/00014 the subject-matter covered by the group, the symbol of which is combined with the symbol of this group, being disclosed without further technical details
• CPC Class Codes: H01L 2924/01012 Magnesium [Mg]
• CPC Class Codes: H01L 2924/01013 Aluminum [Al]
• CPC Class Codes: H01L 2924/01014 Silicon [Si]
• CPC Class Codes: H01L 2924/01019 Potassium [K]
• CPC Class Codes: H01L 2924/0102 Calcium [Ca]
• CPC Class Codes: H01L 2924/01077 Iridium [Ir]
• CPC Class Codes: H01L 2924/01079 : Gold [Au]
• CPC Class Codes: H01L 2924/12041 : LED
• CPC Class Codes: H01L 2924/12043 : Photo diode
• CPC Class Codes: H01L 2924/14 : Integrated circuits

Abstract

A method of, and system for, assaying for selected constituents in liquid mixtures confined by corresponding containing structures having relatively small electromagnetic absorption in at least one transmission wavelength range for transmissions of electromagnetic radiation therethrough, including in vivo assaying for a presence of selected constituents in bloodstreams in circulatory system passageways in mammalian bodies having relatively small electromagnetic radiation absorption in at least one transmission wavelength range for transmissions of electromagnetic radiation between such passageways and outer surfaces of corresponding bodily skin, based on, in the bloodstream example, introducing in a bloodstream a probe comprising a binding base capable of binding to at least one of the selected constituents and of also concurrently conjugating to two different fluorophores of which one has an emission spectra peak distribution in the transmission wavelength range that overlaps an absorption spectra peak distribution of that one remaining. Radiate at least some of the passageways from at least one location on the outer surfaces of the corresponding skin with electromagnetic radiation of wavelengths in the transmission wavelength range and also in an absorption spectra range of that fluorophore having an overlapping emission spectra peak distribution. Detect electromagnetic radiation at least at one location on the outer surfaces of the corresponding skin that has been transmitted from the evaluative radiated passageways of wavelengths in the transmission wavelength range and also in an emission spectra range of that fluorophore having an overlapping absorption spectra peak distribution wherein the emission spectra range differs from the overlapping emission spectra peak distribution.

Claims

1. A method of in vivo assaying for a presence of selected constituents in bloodstreams in circulatory system passageways in mammalian bodies having relatively small electromagnetic radiation absorption in at least one transmission wavelength range for transmissions of electromagnetic radiation between such passageways and outer surfaces of corresponding bodily skin, said method comprising:

introducing in a said bloodstream a probe comprising a binding base capable of binding to at least one of said selected constituents and of also concurrently conjugating to two different fluorophores of which one has an emission spectra peak distribution in said transmission wavelength range that overlaps an absorption spectra peak distribution of that one remaining,

radiating at least some of said passageways, thereby forming evaluative radiated passageways, from at least one location on said outer surfaces of said corresponding skin with evaluation electromagnetic radiation of wavelengths in said transmission wavelength range and also in an absorption spectra range of that said fluorophore having an overlapping emission spectra peak distribution, and

detecting electromagnetic radiation at least at one location on said outer surfaces of said corresponding skin that has been transmitted from said evaluative radiated passageways of wavelengths in said transmission wavelength range and also in an emission spectra range of that said fluorophore having an overlapping absorption spectra peak distribution wherein said emission spectra range differs from said overlapping emission spectra peak distribution.

2. The method of claim 1 further comprising radiating at least some of said passageways, thereby forming survey radiated passageways, with survey electromagnetic radiation of wavelengths in said transmission wavelength range, detecting electromagnetic radiation transmitted from said survey radiated passageways of wavelengths in said transmission wavelength range at plural locations on said outer surfaces of said corresponding skin, and determining that one of said plural locations having a smallest intensity of backscattered electromagnetic radiation relative to those remaining.

3. The method of claim 1 further comprising radiating at least some of said passageways, thereby forming survey radiated passageways, with survey electromagnetic radiation of wavelengths in said transmission wavelength range, detecting electromagnetic radiation transmitted from said survey radiated passageways of wavelengths in said transmission wavelength range using a photodetector having a detecting surface on which said electromagnetic radiation from said survey radiated containing structures impinges, and determining that location on said detecting surface having a largest intensity of impinging electromagnetic radiation.

4. The method of claim 1 wherein said introducing in said bloodstreams of said probe is accomplished through an injector extending through said outer surfaces of corresponding bodily skin into said circulatory system passageways.

5. The method of claim 1 wherein said introducing in said bloodstreams of said probe is accomplished through a source of said probe being positioned against said outer surfaces of corresponding bodily skin to diffuse said probe into said circulatory system passageways.

6. The method of claim 1 wherein said binding base is selected from antibodies and enzymes.

7. The method of claim 1 wherein said radiating of said evaluative radiated passageways with electromagnetic radiation is accomplished through radiating pulses of said electromagnetic radiation at a selected frequency.

8. The method of claim 1 wherein said radiating of said evaluative radiated passageways with electromagnetic radiation is accomplished through radiating electromagnetic radiation of a first selected wavelength and thereafter by radiating electromagnetic radiation of a second selected wavelength.

9. The method of claim 1 wherein said detecting of electromagnetic radiation from said evaluative radiated passageways is accomplished through determining frequencies occurring in changes in intensity of said electromagnetic radiation.

10. The method of claim 1 wherein said detecting of electromagnetic radiation from said evaluative radiated passageways is accomplished through determining of intensity of only a later portion of said electromagnetic radiation that has been detected.

11. The method of claim 1 wherein said electromagnetic radiation detected from said evaluative radiated passageways provides information that is incorporated into signals transmitted to a remote receiver.

12. The method of claim 2 wherein said radiating of said evaluative radiated passageways with electromagnetic radiation is accomplished through radiating electromagnetic radiation of a first selected wavelength and thereafter by radiating electromagnetic radiation of a second selected wavelength.

13. The method of claim 2 wherein said detecting of electromagnetic radiation from said evaluative radiated passageways is accomplished through determining frequencies occurring in changes in intensity of said electromagnetic radiation.

14. The method of claim 3 wherein said radiating of said evaluative radiated passageways with electromagnetic radiation is accomplished through radiating electromagnetic radiation of a first selected wavelength and thereafter by radiating electromagnetic radiation of a second selected wavelength.

15. A method of assaying for a presence of selected constituents in liquid mixtures confined by corresponding containing structures having relatively small electromagnetic radiation absorption in at least one transmission wavelength range for transmissions of electromagnetic radiation therethrough, said method comprising:

introducing in said liquid mixture a probe comprising a binding base capable of binding to at least one of said selected constituents and of also concurrently conjugating to two different fluorophores of which one has an emission spectra peak distribution in said transmission wavelength range that overlaps an absorption spectra peak distribution of that one remaining,

radiating at least some of said containing structures, thereby forming evaluative radiated containing structures, with evaluation electromagnetic radiation of wavelengths in said transmission wavelength range and also in an absorption spectra range of that said fluorophore having an overlapping emission spectra peak distribution, and

detecting electromagnetic radiation that has been transmitted from said evaluative radiated containing structures of wavelengths in said transmission wavelength range and also in an emission spectra range of that said fluorophore having an overlapping absorption spectra peak distribution wherein said emission spectra range differs from said overlapping emission spectra peak distribution.

16. The method of claim 15 further comprising radiating at least some of said containing structures, thereby forming survey radiated containing structures, with survey electromagnetic radiation of wavelengths in said transmission wavelength range, detecting electromagnetic radiation from said survey containing structures of wavelengths in said transmission wavelength range at plural locations across from said surveyed radiated containing structures, and determining that one of said plural locations having an extreme intensity of backscattered electromagnetic radiation relative to those remaining.

17. The method of claim 15 further comprising radiating at least some of said containing structures, thereby forming survey radiated containing structures, with survey electromagnetic radiation of wavelengths in said transmission wavelength range, detecting electromagnetic radiation from said survey containing structures of wavelengths in said transmission wavelength range using a photodetector having a detecting surface on which said electromagnetic radiation from said survey radiated containing structures impinges, and determining that location on said detecting surface having a largest intensity of impinging electromagnetic radiation.

18. A body supported bloodstream biomarker evaluation system for assaying for a presence of selected constituents in bloodstreams in circulatory system passageways in mammalian bodies having relatively small electromagnetic absorption in at least one transmission wavelength range between such passageways and outer surfaces of corresponding bodily skin in which bloodstreams a probe has been introduced comprising a binding base capable of binding to at least one of said selected constituents and of also concurrently conjugating to two different fluorophores of which one has an emission spectra peak distribution in said transmission wavelength range that overlaps an absorption spectra peak distribution of that one remaining, said system comprising:

a substrate,

a first electromagnetic radiation emitter supported by said substrate and capable of emitting evaluation electromagnetic radiation of wavelengths in said transmission wavelength range and also capable of emitting evaluation electromagnetic radiation of wavelengths in an absorption spectra range of that said fluorophore having an overlapping emission spectra peak distribution; and

a first electromagnetic radiation detector supported by said substrate and capable of detecting electromagnetic radiation of wavelengths in said transmission wavelength range and also in an emission spectra range of that said fluorophore having an overlapping absorption spectra peak distribution wherein said emission spectra range differs from said overlapping emission spectra peak distribution.

19. The system of claim 18 further comprising a housing about an interior space therein bounded at least in part by a window side of said housing that is transparent to electromagnetic radiation of wavelengths in said transmission wavelength range, said housing having said substrate therein with said emitter and said detector in said interior space across from said window side.

20. The system of claim 18 further comprising a plurality of electromagnetic radiation emitters, including said first electromagnetic radiation emitter, each supported by said substrate and capable of emitting electromagnetic radiation of wavelengths in said transmission wavelength range, and a plurality of electromagnetic radiation detectors, including said first electromagnetic radiation detector, each supported by said substrate and capable of detecting electromagnetic radiation of wavelengths in said transmission wavelength range.

21. The system of claim 18 further comprising a communications integrated circuit supported by said substrate which is capable of receiving signals related to electromagnetic radiation detected by said first electromagnetic radiation detector and transmitting representations thereof to a remote receiving device.

22. The system of claim 20 wherein members from said plurality of electromagnetic radiation emitters and members from plurality of electromagnetic radiation detectors are located relatively near one another to form survey and evaluation groups and a plurality of said survey and evaluation groups are each positioned at a locations over said substrate differing from one another.