Ruminant mhc class-i-like fc receptors

• US 20060272036A1
• Filed: 02/02/2001
• Published: 11/30/2006
• Est. Priority Date: 02/03/2000
• Status: Abandoned Application

• Current Assignee: Imre Kacskovics, Lennart Hammarstrom
• Original Assignee: Imre Kacskovics, Lennart Hammarstrom
• Inventors: Kacskovics, Imre, Hammarstrom, Lennart
• Application Number:
  US10/181,951
• Publication Number:
  US 20060272036A1
• Time in Patent Office: Days
• US Class Current: 800/7
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A01K 2227/101 Bovine
• CPC Class Codes: A01K 2227/103 Ovine
• CPC Class Codes: A01K 2267/01 Animal expressing industrially exogenous proteins
• CPC Class Codes: C07K 14/70535 Fc-receptors, e.g. CD16, CD32, CD64 (CD2314/705F)
• CPC Class Codes: C07K 14/70539 MHC-molecules, e.g. HLA-molecules
• CPC Class Codes: C07K 16/00 Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002
• CPC Class Codes: C07K 16/283 against Fc-receptors, e.g. CD16, CD32, CD64 CD23 C07K16/2851
• CPC Class Codes: C07K 2317/34 Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
• CPC Class Codes: C07K 2317/52 Constant or Fc region; Isotype
• CPC Class Codes: C12N 15/8509 for producing genetically modified animals, e.g. transgenic

Abstract

Immunoglobulin G (IgG) transporting ruminant Fc receptor (FcRn) α-chain DNA molecules, especially those of cow, dromedary and sheep (SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3) are disclosed. Protein expressed by said FcRn α-chain DNA molecules are disclosed and include SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6. Vectors containing the ruminant IgG transporting FcRn α-chain DNA molecules, and hosts transformed with such vectors are also included. Further, a method of producing colostrums or milk with enhanced levels of immunoglobulins or proteins fused to immunoglobulin γ-chains of FcRn interacting parts thereof is also disclosed.

Claims

1. A method of producing colostrums or milk with enhanced levels of immunoglobulins or proteins fused to immunoglobulin γ

-chains or FcRn interacting parts thereof, comprising the steps of transferring an immunoglobulin G (IgG) transporting ruminant Fc receptor (FcRn) α

-chain DNA molecule through transient or persistent transgenesis into the corresponding ruminant animal for overexpression of the protein expressed by the ruminant FcRn α

-chain DNA molecule, optionally at concomitant upregulation of the expression of the corresponding β

2-microglobulin gene, to increase the number of functional receptors in the udder, thereby enhancing the transport of immunoglobulins and/or proteins fused to immunoglobulin γ

-chains or FcRn interacting parts thereof from, or through, the udder into the colostrums or milk.

2. The method according to claim 1, wherein the ruminant of the immunoglobulin G (IgG) transporting ruminant Fc receptor (FcRn) α

-chain DNA molecule is selected from the group consisting of cow, dromedary and sheep.

3. The method according to claim 2, wherein the DNA molecule has a nucleotide sequence selected from the group consisting of SEQ ID NO:

1, SEQ ID NO;

2, SEQ ID NO;

3, and modified sequences of these three sequences expressing proteins with IgG transporting function.

4. The method according to claim 1, wherein the ruminant of the protein expressed by the ruminant FcRn α

-chain DNA molecule is selected from the group consisting of cow, dromedary and sheep.

5. The method according to claim 4, wherein the protein has an amino acid sequence selected from the group consisting of SEQ ID NO:

4, SEQ ID NO;

5, SEQ ID NO;

6, and modified sequences of these three sequences with IgG transporting function.