Detecting element and detection method

• US 20060272433A1
• Filed: 01/18/2005
• Published: 12/07/2006
• Est. Priority Date: 01/23/2004
• Status: Active Grant

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Yamamichi, Junta
• Granted Patent: US 7,863,051 B2
• Time in Patent Office: Days
• US Class Current: 73/864.710
• CPC Class Codes: B01L 2200/0636 Focussing flows, e.g. to laminate flows
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/502776 specially adapted for focusing or laminating flows
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 2021/6482 Sample cells, cuvettes
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/25625 Dilution
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A detecting element for detecting a plurality of different substances in a specimen, characterized by including: a channel provided on a substrate, which is capable of forming a plurality of layers of flow of a fluid; and a plurality of different substance trapping portions provided in the channel, for trapping the plurality of different substances in the specimen, respectively, in which: the plurality of different substance trapping portions are provided separately in accordance with the plurality of layers of flow of a fluid to be formed; and the plurality of different substance trapping portions are each arranged to acquire independent information on each of the substances in the specimen through an action between the fluid and the trapped substance.

Claims

1. A detecting element for detecting a plurality of different substances in a specimen, characterized by comprising:

a channel provided on a substrate, which is capable of forming a plurality of layers of flow of a fluid; and

a plurality of different substance trapping portions provided in the channel, for trapping the plurality of different substances in the specimen, respectively, wherein;

the plurality of different substance trapping portions are provided separately in accordance with the plurality of layers of flow of a fluid to be formed; and

the plurality of different substance trapping portions are each arranged to acquire independent information on each of the substances in the specimen through an action between the fluid and the trapped substance.

2. A detecting element according to claim 1, characterized by further comprising a detecting unit for detecting the information.

3. A detecting element according to claim 1 or 2, characterized in that:

the plurality of different substances in the specimen each have a label; and

the fluid flowing along the plurality of layers of flow comprises a fluid acting on the label to discharge an active product.

4. A detecting element according to claim 3, characterized in that the label comprises a substance having a catalytic action, a substance having electrochemiluminescence, or a fluorescent substance.

5. A detecting element according to claim 4, wherein the substance having a catalytic action is selected from glucose oxidase, choline oxidase, lactose oxidase, and horseradish peroxidase.

6. A detecting element according to claim 1, characterized in that an antibody is immobilized on each of the substance trapping portions.

7. A detection method for detecting a plurality of different substances in a specimen, characterized by comprising the steps of:

introducing the specimen into a channel having a plurality of different substance trapping portions for specifically trapping the plurality of different substances, respectively, to trap the substances in the substance trapping portions;

forming a plurality of layers of flow of a fluid in the channel; and

switching and passing the fluid forming the plurality of layers of flow, to acquire independent information on each of the substances in the specimen through an action between the fluid and the trapped substance.

8. A detection method according to claim 7, wherein the plurality of different substances in the specimen each have a label.

9. A detection method according to claim 8, wherein the label employs a substance having a catalytic action, a substance having electrochemiluminescence, or a fluorescent substance.

10. A detection method according to claim 7, wherein the plurality of different substances in the specimen are selected from a biological substance, allergen, bacteria, and virus.

11. A detection method according to claim 10, wherein the biological substance is selected from a protein, a nucleic acid, and a sugar chain.