Enhancement of nucleic acid amplification using double-stranded DNA binding proteins

• US 20060275792A1
• Filed: 02/28/2006
• Published: 12/07/2006
• Est. Priority Date: 11/15/2004
• Status: Abandoned Application

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Javorschi-Miller, Sandrine M. M., Lee, Jun E., Potter, Robert J.
• Application Number:
  US11/365,383
• Publication Number:
  US 20060275792A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6848 characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 2525/15 incorporating a consensus or conserved sequence
• CPC Class Codes: C12Q 2535/125 Allele specific primer extension
• CPC Class Codes: C12Q 2563/107 fluorescence

Abstract

Disclosed are methods and materials for enhancing the efficiency, specificity and/or specificity of nucleic acid amplification reactions (e.g., PCR amplification reactions). The disclosed inventions involve the use of nonspecific double-stranded DNA binding proteins in amplification reactions. The methods described herein are useful in a wide variety of molecular biology applications including allele specific PCR.

Claims

1. A method for amplification of a DNA molecule, comprising contacting said DNA molecule with a thermostable nucleic acid polymerase and a nonspecific double-stranded DNA binding protein, whereby said nucleic acid molecule is amplified.

2. A composition comprising a nucleic acid molecule, a thermostable nucleic acid polymerase and a nonspecific double-stranded DNA binding protein.