Enhancement of nucleic acid amplification using double-stranded DNA binding proteins
First Claim
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1. A method for amplification of a DNA molecule, comprising contacting said DNA molecule with a thermostable nucleic acid polymerase and a nonspecific double-stranded DNA binding protein, whereby said nucleic acid molecule is amplified.
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Abstract
Disclosed are methods and materials for enhancing the efficiency, specificity and/or specificity of nucleic acid amplification reactions (e.g., PCR amplification reactions). The disclosed inventions involve the use of nonspecific double-stranded DNA binding proteins in amplification reactions. The methods described herein are useful in a wide variety of molecular biology applications including allele specific PCR.
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2 Claims
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1. A method for amplification of a DNA molecule, comprising contacting said DNA molecule with a thermostable nucleic acid polymerase and a nonspecific double-stranded DNA binding protein, whereby said nucleic acid molecule is amplified.
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2. A composition comprising a nucleic acid molecule, a thermostable nucleic acid polymerase and a nonspecific double-stranded DNA binding protein.
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