Use of a fluorescent protein for detecting interactions between a target protein and its ligand
First Claim
1. A process for identifying and/or quantifying interactions between a receptor and a non-fluorescent molecule which is biologically active with respect to said receptor, wherein said non-fluorescent molecule forms a reversible non-covalent interaction with said receptor, comprising adding a biologically active non-fluorescent molecule to cells, or cell fragments that express DNA coding for a receptor labeled with fluorescent protein and for a labeled G protein, characterized in that:
- i) an agonist and biologically active non-fluorescent molecule triggers a signal transduction detected by a variation in the energy transfer between the receptor labeled with the fluorescent protein and the G protein labeled with the label;
or ii) an antagonistic biologically active non-fluorescent molecule inhibits the signal transduction brought about by an agonist and detected by variation in the transfer of fluorescence energy between the receptor labeled with the fluorescent protein and the G protein labeled with the label.
1 Assignment
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Accused Products
Abstract
A fluorescent protein selected in particular among the autofluorescent proteins for detecting the non-covalent interaction between a target protein marked by the fluorescent protein and one of its ligands marked by a marker consisting of: either a molecule capable of absorbing the light emitted by the fluorescent protein, or a fluorescent substance, the detection taking place by fluorescence energy transfer: between the fluorescent protein and the fluorescent substance, the fluorescent substance being such that it is excitable at the fluorescent protein emitting wavelength, or it emits at the fluorescent protein emitting wavelength; between the fluorescent protein and the molecule capable of absorbing the light emitted by the fluorescent protein.
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Citations
7 Claims
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1. A process for identifying and/or quantifying interactions between a receptor and a non-fluorescent molecule which is biologically active with respect to said receptor, wherein said non-fluorescent molecule forms a reversible non-covalent interaction with said receptor, comprising adding a biologically active non-fluorescent molecule to cells, or cell fragments that express DNA coding for a receptor labeled with fluorescent protein and for a labeled G protein, characterized in that:
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i) an agonist and biologically active non-fluorescent molecule triggers a signal transduction detected by a variation in the energy transfer between the receptor labeled with the fluorescent protein and the G protein labeled with the label;
orii) an antagonistic biologically active non-fluorescent molecule inhibits the signal transduction brought about by an agonist and detected by variation in the transfer of fluorescence energy between the receptor labeled with the fluorescent protein and the G protein labeled with the label. - View Dependent Claims (2, 3, 4, 5, 6, 7)
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Specification