Enzyme sensor including a water-containing spacer layer
First Claim
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1. An amperometric enzyme sensor for determining the concentration of an analyte in a fluid sample, comprising an electrode, a water-containing spacer layer in contact with said electrode, at least one intermediate layer, the innermost of said at least one intermediate layer being in contact with said spacer layer, and at least one enzyme layer, the innermost of said at least one enzyme layer being in contact with the outermost of said intermediate layer(s).
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Abstract
The present disclosure relates to an amperometric enzyme sensor including a water-containing spacer layer in contact with an electrode. The sensor is useful determining the presence or amount of biological analytes, e.g. glucose, lactate, creatine, creatinine, etc.
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20 Claims
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1. An amperometric enzyme sensor for determining the concentration of an analyte in a fluid sample, comprising
an electrode, a water-containing spacer layer in contact with said electrode, at least one intermediate layer, the innermost of said at least one intermediate layer being in contact with said spacer layer, and at least one enzyme layer, the innermost of said at least one enzyme layer being in contact with the outermost of said intermediate layer(s).
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15. An amperometric enzyme sensor for determining the concentration of creatine in a fluid sample, comprising
a metal electrode, a water-containing spacer layer in contact with said metal electrode, an interference limiting layer in contact with said spacer layer, an enzyme layer comprising sarcosine oxidase and creatinase in contact with said interference limiting layer, and a cover membrane layer for said enzyme layer, wherein said cover membrane layer comprises a porous polyethylene terephthalate material, and wherein the outer surface and pore mouths of at least one face of the porous polyethylene terephthalate material are covered by a hydrophilic polyurethane comprising backbone segments of polyethylene glycol in a weight ratio of polyethylene glycol segments of at least about 5% (w/w) and/or have a water content when wetted of at least about 25% (w/w).
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17. An amperometric enzyme sensor for determining the concentration of creatinine in a fluid sample, comprising
a metal electrode, a water-containing spacer layer in contact with said metal electrode, an interference limiting layer in contact with said spacer layer, an enzyme layer comprising sarcosine oxidase, creatininase and creatinase in contact with said interference limiting layer, and a cover membrane layer for said enzyme layer, wherein said cover membrane layer comprises a porous polyethylene terephthalate material, and wherein the outer surface and pore mouths of at least one face of the porous polyethylene terephthalate material are covered by a hydrophilic polyurethane comprising backbone segments of polyethylene glycol in a weight ratio of polyethylene glycol segments of at least about 5% (w/w) and/or have a water content when wetted of at least about 25% (w/w).
Specification