Mesoderm and definitive endoderm cell populations
First Claim
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1. A cell population enriched for mesendoderm and mesoderm cells.
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Abstract
The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. The cell populations of the invention are useful for generating cells for cell replacement therapy.
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Citations
30 Claims
- 1. A cell population enriched for mesendoderm and mesoderm cells.
- 11. A cell population enriched for endoderm cells.
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20. A method of isolating a cell population enriched for mesoderm cells comprising culturing embryonic stem cells in the absence of serum and in the presence of activin in an amount sufficient to induce differentiation of embryonic stem cells to mesoderm but insufficient to induce differentiation of embryonic stems cells to endoderm, and isolating cells that express brachyury.
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25. A method of identifying genes involved in cell differentiation comprising incubating embryonic stem cells under conditions to obtain cells that express brachyury, isolating two populations of cells that express brachyury after two different amounts of time in culture, comparing gene expression profiles in said two populations, and identifying genes that are uniquely expressed in a population, wherein identification of a gene that is uniquely expressed in a population is indicative of identification of a gene involved in cell differentiation.
- 26. A method of making antibodies that recognize cells that express brachyury but not cells that do not express brachyury comprising inserting a selectable marker into the brachyury locus of embryonic stem cells, culturing said cells, isolating cells that express brachyury by selecting for cells that express the selectable marker to provide an isolated population of cells that express brachyury, and raising antibodies against said isolated population.
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30. A transgenic non-human mammal having a genome comprising a transgene in which DNA encoding a selectable marker is present in the brachyury locus and the selectable marker is expressed in cells that express brachyury.
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