INDUSTRIAL BIOSYNTHESIZER SYSTEM AND METHOD

• US 20060280651A1
• Filed: 04/18/2006
• Published: 12/14/2006
• Est. Priority Date: 04/18/2005
• Status: Abandoned Application

• Current Assignee: Asahi Kasei Bioprocess, Inc. (Asahi Kasei Corporation)
• Original Assignee: Asahi Kasei Bioprocess, Inc. (Asahi Kasei Corporation)
• Inventors: Walker, John, Sanderson, James, Bellafiore, Louis
• Application Number:
  US11/379,114
• Publication Number:
  US 20060280651A1
• Time in Patent Office: Days
• US Class Current: 422/62
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00286 Reactor vessels with top and bottom openings
• CPC Class Codes: B01J 2219/00398 in multiple arrangements
• CPC Class Codes: B01J 2219/00423 using filtration, e.g. through porous frits
• CPC Class Codes: B01J 2219/00477 Means for pressurising the reaction vessels
• CPC Class Codes: B01J 2219/00479 Means for mixing reactants or products in the reaction vessels
• CPC Class Codes: B01J 2219/00481 by the use of moving stirrers within the reaction vessels
• CPC Class Codes: B01J 2219/00497 Features relating to the solid phase supports
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00599 Solution-phase processes
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00689 using computers
• CPC Class Codes: B01J 2219/00698 Measurement and control of process parameters
• CPC Class Codes: B01J 2219/00707 separated from the reactor apparatus
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides

Abstract

A synthesizer system for use as either a freestanding or facility integrated device and a method of use. The system includes an inlet manifold of diaphragm valves that receives at least two liquid feeds. The streams flow either to a blending module or directly to a delivery module. From there they are delivered to a reactor for the sequential creation of desired compounds. For flow through solid phase synthesis added capability for feed recirculation and effluent detection with feedback control is included.

Claims

1. An automated compound synthesis system comprising:

a) an inlet module featuring a manifold having an outlet and a plurality of diaphragm valves, each of said diaphragm valves having an inlet adapted to communicate with an external feed of a building block or reagent; and

b) a delivery module in communication with the outlet of the manifold of the inlet module and receiving a feed stream therefrom, said delivery module adapted to communicate with a reaction vessel to deliver the feed stream to the reaction vessel.

2. The synthesis system of claim 1 wherein the diaphragm valves are multi-port cluster diaphragm valves.

3. The synthesis system of claim 1 wherein the diaphragm valves are multi-port zero static diaphragm valves.

4. The synthesis system of claim 1 further comprising a blending module in circuit between the delivery module and the reaction vessel.

5. The synthesis system of claim 1 further comprising a flow control module in circuit between the delivery module and the reaction vessel.

6. The synthesis system of claim 1 further comprising a PAT detection module in circuit between the delivery module and the reaction vessel.

7. The synthesis system of claim 4 wherein the blending module includes a valve or valves.

8. The synthesis system of claim 1 further comprising a cart provided with rollers upon which the system is mounted so that the system may be rolled across a surface.

9. A method for providing synthesis capability to a laboratory, pilot or commercial scale system including the steps of:

a) connecting at least two liquid feeds to a synthesizer and passing them through diaphragm valves arranged in a manifold; and

b) delivering the liquid feeds from the manifold to a reaction vessel.

10. The method of claim 9 further comprising the step of controlling the delivery of the liquid feeds to the reaction vessel by passing them through a flow control module.

11. The method of claim 9 further comprising the step of blending the feed streams with a blending module so that a blended liquid stream of feeds is produced prior the delivery to a reaction vessel.

12. The method of claim 9 further comprising the steps of detecting a composition of the feed liquid stream via a detection module and generating a corresponding signal and receiving the signal with the controller and controlling the inlet valve and/or delivery module with the controller based upon the received signal.

13. The method of claim 9 wherein the reaction vessel is a flow-through reactor.

14. The method of claim 13 further comprising the steps of

a) re-circulating of the reagents and/or reactants through the reactor;

b) receiving the effluent from the reaction vessel displaced by incoming feeds or otherwise;

c) using a post-reactor detection module to determine the completeness of reaction based on the detected signal for the reagent or reactant used in a step;

d) using a controller to determine whether to continue re-circulation, adjust the rate of re-circulation or stop the re-circulation; and

e) using the controller to determine whether to begin the next step or pause the system.

15. The method of claim 9 wherein the synthesizer inlet valve(s) are multi-port cluster diaphragm valves.

16. The method of claim 9 wherein the synthesizer inlet valve(s) are of a zero static design to reduce carryover and/or mixing of feed streams.

17. The method of claim 9 wherein the synthesizer inlet valve(s) are of a zero static design connected into a low dead volume valve assembly to provide the highest level of reduced carryover and/or mixing of feed streams.